This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1py0
From Proteopedia
(New page: 200px<br /><applet load="1py0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1py0, resolution 2.00Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1py0.gif|left|200px]]<br /><applet load="1py0" size=" | + | [[Image:1py0.gif|left|200px]]<br /><applet load="1py0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1py0, resolution 2.00Å" /> | caption="1py0, resolution 2.00Å" /> | ||
'''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''<br /> | '''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been | + | A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been developed for the characterisation of proteins by paramagnetic NMR spectroscopy. The probe consists of a lanthanide chelated by a derivative of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive functional groups. The CLaNP molecule is attached to a protein by two engineered, surface-exposed, Cys residues in a bidentate manner. This drastically limits the dynamics of the metal relative to the protein and enables measurements of pseudocontact shifts. NMR spectroscopy experiments on a diamagnetic control and the crystal structure of the probe-protein complex demonstrate that the protein structure is not affected by probe attachment. The probe is able to induce pseudocontact shifts to at least 40 A from the metal and causes residual dipolar couplings due to alignment at a high magnetic field. The molecule exists in several isomeric forms with different paramagnetic tensors; this provides a fast way to obtain long-range distance restraints. |
==About this Structure== | ==About this Structure== | ||
| - | 1PY0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with ZN, Y1, SO4 and YMA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1PY0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=Y1:'>Y1</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=YMA:'>YMA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PY0 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Alcaligenes faecalis]] | [[Category: Alcaligenes faecalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Boulanger, M | + | [[Category: Boulanger, M J.]] |
| - | [[Category: Hupkes, H | + | [[Category: Hupkes, H J.]] |
[[Category: Impagliazzo, A.]] | [[Category: Impagliazzo, A.]] | ||
[[Category: Kosters, W.]] | [[Category: Kosters, W.]] | ||
| - | [[Category: Murphy, M | + | [[Category: Murphy, M E.]] |
| - | [[Category: Peters, J | + | [[Category: Peters, J A.]] |
[[Category: Prudencio, M.]] | [[Category: Prudencio, M.]] | ||
[[Category: Rohovec, J.]] | [[Category: Rohovec, J.]] | ||
| Line 30: | Line 30: | ||
[[Category: nmr probe]] | [[Category: nmr probe]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:43 2008'' |
Revision as of 12:33, 21 February 2008
|
Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe
Overview
A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been developed for the characterisation of proteins by paramagnetic NMR spectroscopy. The probe consists of a lanthanide chelated by a derivative of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive functional groups. The CLaNP molecule is attached to a protein by two engineered, surface-exposed, Cys residues in a bidentate manner. This drastically limits the dynamics of the metal relative to the protein and enables measurements of pseudocontact shifts. NMR spectroscopy experiments on a diamagnetic control and the crystal structure of the probe-protein complex demonstrate that the protein structure is not affected by probe attachment. The probe is able to induce pseudocontact shifts to at least 40 A from the metal and causes residual dipolar couplings due to alignment at a high magnetic field. The molecule exists in several isomeric forms with different paramagnetic tensors; this provides a fast way to obtain long-range distance restraints.
About this Structure
1PY0 is a Single protein structure of sequence from Alcaligenes faecalis with , , and as ligands. Full crystallographic information is available from OCA.
Reference
A caged lanthanide complex as a paramagnetic shift agent for protein NMR., Prudencio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M, Chemistry. 2004 Jul 5;10(13):3252-60. PMID:15224334
Page seeded by OCA on Thu Feb 21 14:33:43 2008
Categories: Alcaligenes faecalis | Single protein | Boulanger, M J. | Hupkes, H J. | Impagliazzo, A. | Kosters, W. | Murphy, M E. | Peters, J A. | Prudencio, M. | Rohovec, J. | Tocheva, E. | Ubbink, M. | SO4 | Y1 | YMA | ZN | Cupredoxin | Nmr probe
