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1qrr
From Proteopedia
(New page: 200px<br /><applet load="1qrr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qrr, resolution 1.6Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1qrr.gif|left|200px]]<br /><applet load="1qrr" size=" | + | [[Image:1qrr.gif|left|200px]]<br /><applet load="1qrr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qrr, resolution 1.6Å" /> | caption="1qrr, resolution 1.6Å" /> | ||
'''CRYSTAL STRUCTURE OF SQD1 PROTEIN COMPLEX WITH NAD AND UDP-GLUCOSE'''<br /> | '''CRYSTAL STRUCTURE OF SQD1 PROTEIN COMPLEX WITH NAD AND UDP-GLUCOSE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The SQD1 enzyme is believed to be involved in the biosynthesis of the | + | The SQD1 enzyme is believed to be involved in the biosynthesis of the sulfoquinovosyl headgroup of plant sulfolipids, catalyzing the transfer of SO(3)(-) to UDP-glucose. We have determined the structure of the complex of SQD1 from Arabidopsis thaliana with NAD(+) and the putative substrate UDP-glucose at 1.6-A resolution. Both bound ligands are completely buried within the binding cleft, along with an internal solvent cavity which is the likely binding site for the, as yet, unidentified sulfur-donor substrate. SQD1 is a member of the short-chain dehydrogenase/reductase (SDR) family of enzymes, and its structure shows a conservation of the SDR catalytic residues. Among several highly conserved catalytic residues, Thr-145 forms unusually short hydrogen bonds with both susceptible hydroxyls of UDP-glucose. A His side chain may also be catalytically important in the sulfonation. |
==About this Structure== | ==About this Structure== | ||
| - | 1QRR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with SO4, NAD and UPG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1QRR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NAD:'>NAD</scene> and <scene name='pdbligand=UPG:'>UPG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QRR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Benning, C.]] | [[Category: Benning, C.]] | ||
[[Category: Essigmann, B.]] | [[Category: Essigmann, B.]] | ||
| - | [[Category: Garavito, R | + | [[Category: Garavito, R M.]] |
| - | [[Category: Mulichak, A | + | [[Category: Mulichak, A M.]] |
| - | [[Category: Theisen, M | + | [[Category: Theisen, M J.]] |
[[Category: NAD]] | [[Category: NAD]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
| Line 25: | Line 25: | ||
[[Category: short hydrogen bonds]] | [[Category: short hydrogen bonds]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:42:57 2008'' |
Revision as of 12:43, 21 February 2008
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CRYSTAL STRUCTURE OF SQD1 PROTEIN COMPLEX WITH NAD AND UDP-GLUCOSE
Overview
The SQD1 enzyme is believed to be involved in the biosynthesis of the sulfoquinovosyl headgroup of plant sulfolipids, catalyzing the transfer of SO(3)(-) to UDP-glucose. We have determined the structure of the complex of SQD1 from Arabidopsis thaliana with NAD(+) and the putative substrate UDP-glucose at 1.6-A resolution. Both bound ligands are completely buried within the binding cleft, along with an internal solvent cavity which is the likely binding site for the, as yet, unidentified sulfur-donor substrate. SQD1 is a member of the short-chain dehydrogenase/reductase (SDR) family of enzymes, and its structure shows a conservation of the SDR catalytic residues. Among several highly conserved catalytic residues, Thr-145 forms unusually short hydrogen bonds with both susceptible hydroxyls of UDP-glucose. A His side chain may also be catalytically important in the sulfonation.
About this Structure
1QRR is a Single protein structure of sequence from Arabidopsis thaliana with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of SQD1, an enzyme involved in the biosynthesis of the plant sulfolipid headgroup donor UDP-sulfoquinovose., Mulichak AM, Theisen MJ, Essigmann B, Benning C, Garavito RM, Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13097-102. PMID:10557279
Page seeded by OCA on Thu Feb 21 14:42:57 2008
