1qwc
From Proteopedia
(New page: 200px<br /><applet load="1qwc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qwc, resolution 2.3Å" /> '''Rat neuronal nitric o...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1qwc.jpg|left|200px]]<br /><applet load="1qwc" size=" | + | [[Image:1qwc.jpg|left|200px]]<br /><applet load="1qwc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qwc, resolution 2.3Å" /> | caption="1qwc, resolution 2.3Å" /> | ||
'''Rat neuronal nitric oxide synthase oxygenase domain in complex with W1400 inhibitor.'''<br /> | '''Rat neuronal nitric oxide synthase oxygenase domain in complex with W1400 inhibitor.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The high level of amino acid conservation and structural similarity in the | + | The high level of amino acid conservation and structural similarity in the immediate vicinity of the substrate binding sites of the oxygenase domains of the nitric-oxide synthase (NOS) isoforms (eNOSoxy, iNOSoxy, and nNOSoxy) make the interpretation of the structural basis of inhibitor isoform specificity a challenge and provide few clues for the design of new selective compounds. Crystal structures of iNOSoxy and nNOSoxy complexed with the inhibitors W1400 and Nomega-propyl-l-arginine provide a rationale for their isoform specificity. It involves differences outside the immediate active site as well as a conformational flexibility in the active site that allows the adoption of distinct conformations in response to interactions with the inhibitors. This flexibility is determined by isoform-specific residues outside the active site. |
==About this Structure== | ==About this Structure== | ||
| - | 1QWC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ZN, HEM, H4B and 14W as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http:// | + | 1QWC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=H4B:'>H4B</scene> and <scene name='pdbligand=14W:'>14W</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWC OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Fedorov, R.]] | [[Category: Fedorov, R.]] | ||
| - | [[Category: Ghosh, D | + | [[Category: Ghosh, D K.]] |
[[Category: Hartmann, E.]] | [[Category: Hartmann, E.]] | ||
[[Category: Schlichting, I.]] | [[Category: Schlichting, I.]] | ||
| Line 24: | Line 24: | ||
[[Category: rat nnosoxy w1400 inhibitor complex]] | [[Category: rat nnosoxy w1400 inhibitor complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:24 2008'' |
Revision as of 12:44, 21 February 2008
|
Rat neuronal nitric oxide synthase oxygenase domain in complex with W1400 inhibitor.
Overview
The high level of amino acid conservation and structural similarity in the immediate vicinity of the substrate binding sites of the oxygenase domains of the nitric-oxide synthase (NOS) isoforms (eNOSoxy, iNOSoxy, and nNOSoxy) make the interpretation of the structural basis of inhibitor isoform specificity a challenge and provide few clues for the design of new selective compounds. Crystal structures of iNOSoxy and nNOSoxy complexed with the inhibitors W1400 and Nomega-propyl-l-arginine provide a rationale for their isoform specificity. It involves differences outside the immediate active site as well as a conformational flexibility in the active site that allows the adoption of distinct conformations in response to interactions with the inhibitors. This flexibility is determined by isoform-specific residues outside the active site.
About this Structure
1QWC is a Single protein structure of sequence from Rattus norvegicus with , , and as ligands. Active as Nitric-oxide synthase, with EC number 1.14.13.39 Full crystallographic information is available from OCA.
Reference
Structural basis for the specificity of the nitric-oxide synthase inhibitors W1400 and Nomega-propyl-L-Arg for the inducible and neuronal isoforms., Fedorov R, Hartmann E, Ghosh DK, Schlichting I, J Biol Chem. 2003 Nov 14;278(46):45818-25. Epub 2003 Sep 3. PMID:12954642
Page seeded by OCA on Thu Feb 21 14:44:24 2008
