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1r7r
From Proteopedia
(New page: 200px<br /> <applet load="1r7r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r7r, resolution 3.60Å" /> '''The crystal structu...) |
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| - | [[Image:1r7r.gif|left|200px]]<br /> | + | [[Image:1r7r.gif|left|200px]]<br /><applet load="1r7r" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1r7r" size=" | + | |
caption="1r7r, resolution 3.60Å" /> | caption="1r7r, resolution 3.60Å" /> | ||
'''The crystal structure of murine p97/VCP at 3.6A'''<br /> | '''The crystal structure of murine p97/VCP at 3.6A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | p97/VCP is a member of the AAA ATPase family and has roles in both | + | p97/VCP is a member of the AAA ATPase family and has roles in both membrane fusion and ubiquitin dependent protein degradation. Here, we present a 3.6A crystal structure of murine p97 in which D2 domain has been modelled as poly-alanine and the remaining approximately 100 residues are absent. The resulting structure illustrates a head-to-tail packing arrangement of the two p97 AAA domains in a natural hexameric state with D1 ADP bound and D2 nucleotide free. The head-to-tail packing arrangement observed in this structure is in contrast to our previously predicted tail-to-tail packing model. The linker between the D1 and D2 domains is partially disordered, suggesting a flexible nature. Normal mode analysis of the crystal structure suggests anti-correlated motions and distinct conformational states of the two AAA domains. |
==About this Structure== | ==About this Structure== | ||
| - | 1R7R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1R7R with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http:// | + | 1R7R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1R7R with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R7R OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Beuron, F.]] | [[Category: Beuron, F.]] | ||
| - | [[Category: Briggs, L | + | [[Category: Briggs, L C.]] |
| - | [[Category: Flynn, T | + | [[Category: Flynn, T C.]] |
| - | [[Category: Freemont, P | + | [[Category: Freemont, P S.]] |
[[Category: Huyton, T.]] | [[Category: Huyton, T.]] | ||
[[Category: Kondo, H.]] | [[Category: Kondo, H.]] | ||
[[Category: Ma, J.]] | [[Category: Ma, J.]] | ||
| - | [[Category: Pye, V | + | [[Category: Pye, V E.]] |
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
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[[Category: vcp]] | [[Category: vcp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:08 2008'' |
Revision as of 12:48, 21 February 2008
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The crystal structure of murine p97/VCP at 3.6A
Overview
p97/VCP is a member of the AAA ATPase family and has roles in both membrane fusion and ubiquitin dependent protein degradation. Here, we present a 3.6A crystal structure of murine p97 in which D2 domain has been modelled as poly-alanine and the remaining approximately 100 residues are absent. The resulting structure illustrates a head-to-tail packing arrangement of the two p97 AAA domains in a natural hexameric state with D1 ADP bound and D2 nucleotide free. The head-to-tail packing arrangement observed in this structure is in contrast to our previously predicted tail-to-tail packing model. The linker between the D1 and D2 domains is partially disordered, suggesting a flexible nature. Normal mode analysis of the crystal structure suggests anti-correlated motions and distinct conformational states of the two AAA domains.
About this Structure
1R7R is a Single protein structure of sequence from Mus musculus with as ligand. The following page contains interesting information on the relation of 1R7R with [AAA+ Proteases]. Full crystallographic information is available from OCA.
Reference
The crystal structure of murine p97/VCP at 3.6A., Huyton T, Pye VE, Briggs LC, Flynn TC, Beuron F, Kondo H, Ma J, Zhang X, Freemont PS, J Struct Biol. 2003 Dec;144(3):337-48. PMID:14643202
Page seeded by OCA on Thu Feb 21 14:48:08 2008
Categories: AAA+ Proteases | Mus musculus | Single protein | Beuron, F. | Briggs, L C. | Flynn, T C. | Freemont, P S. | Huyton, T. | Kondo, H. | Ma, J. | Pye, V E. | Zhang, X. | ADP | Aaa | Cdc48 | P97 | Vcp
