1ryk
From Proteopedia
(New page: 200px<br /><applet load="1ryk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ryk" /> '''Solution NMR Structure Protein yjbJ from Esc...) |
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| - | [[Image:1ryk.gif|left|200px]]<br /><applet load="1ryk" size=" | + | [[Image:1ryk.gif|left|200px]]<br /><applet load="1ryk" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ryk" /> | caption="1ryk" /> | ||
'''Solution NMR Structure Protein yjbJ from Escherichia coli. Northeast Structural Genomics Consortium Target ET93; Ontario Centre for Structural Proteomics target EC0298_1_69;'''<br /> | '''Solution NMR Structure Protein yjbJ from Escherichia coli. Northeast Structural Genomics Consortium Target ET93; Ontario Centre for Structural Proteomics target EC0298_1_69;'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The influx of genomic sequence information has led to the concept of | + | The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1RYK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1RYK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
| - | [[Category: Cort, J | + | [[Category: Cort, J R.]] |
| - | [[Category: Edwards, A | + | [[Category: Edwards, A M.]] |
| - | [[Category: Kennedy, M | + | [[Category: Kennedy, M A.]] |
[[Category: Liao, J.]] | [[Category: Liao, J.]] | ||
| - | [[Category: NESG, Northeast | + | [[Category: NESG, Northeast Structural Genomics Consortium.]] |
[[Category: Pineda-Lucena, A.]] | [[Category: Pineda-Lucena, A.]] | ||
[[Category: Wu, B.]] | [[Category: Wu, B.]] | ||
| Line 30: | Line 30: | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:55 2008'' |
Revision as of 12:55, 21 February 2008
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Solution NMR Structure Protein yjbJ from Escherichia coli. Northeast Structural Genomics Consortium Target ET93; Ontario Centre for Structural Proteomics target EC0298_1_69;
Overview
The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.
About this Structure
1RYK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
An NMR approach to structural proteomics., Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485
Page seeded by OCA on Thu Feb 21 14:55:55 2008
Categories: Escherichia coli | Single protein | Arrowsmith, C H. | Cort, J R. | Edwards, A M. | Kennedy, M A. | Liao, J. | NESG, Northeast Structural Genomics Consortium. | Pineda-Lucena, A. | Wu, B. | Yee, A. | Alpha protein | Nesg | Northeast structural genomics consortium | Ocsp | Protein structure initiative | Psi | Structural genomics
