1sd5
From Proteopedia
(New page: 200px<br /><applet load="1sd5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sd5, resolution 1.68Å" /> '''Crystal structure of...) |
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| - | [[Image:1sd5.gif|left|200px]]<br /><applet load="1sd5" size=" | + | [[Image:1sd5.gif|left|200px]]<br /><applet load="1sd5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1sd5, resolution 1.68Å" /> | caption="1sd5, resolution 1.68Å" /> | ||
'''Crystal structure of Rv1626'''<br /> | '''Crystal structure of Rv1626'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We describe the crystal structure of Rv1626 from Mycobacterium | + | We describe the crystal structure of Rv1626 from Mycobacterium tuberculosis at 1.48 A resolution and the corresponding solution structure determined from small angle X-ray scattering. The N-terminal domain shows structural homology to the receiver domains found in bacterial two-component systems. The C-terminal domain has high structural homology to a recently discovered RNA binding domain involved in transcriptional antitermination. The molecule in solution was found to be monomeric as it is in the crystal, but in solution it undergoes a conformational change that is triggered by changes in ionic strength. This is the first structure that links the phosphorylation cascade of the two-component systems with the antitermination event in the transcriptional machinery. Rv1626 belongs to a family of proteins, which we propose calling phosphorylation-dependent transcriptional antitermination regulators, so far only found in bacteria, and includes NasT, a protein from the assimilatory nitrate/nitrite reductase operon of Azetobacter vinelandii. |
==About this Structure== | ==About this Structure== | ||
| - | 1SD5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv] with IOD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1SD5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv] with <scene name='pdbligand=IOD:'>IOD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SD5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Feng, V.]] | [[Category: Feng, V.]] | ||
| - | [[Category: Morth, J | + | [[Category: Morth, J P.]] |
| - | [[Category: Perry, L | + | [[Category: Perry, L J.]] |
| - | [[Category: Svergun, D | + | [[Category: Svergun, D I.]] |
| - | [[Category: TBSGC, TB | + | [[Category: TBSGC, TB Structural Genomics Consortium.]] |
| - | [[Category: Tucker, P | + | [[Category: Tucker, P A.]] |
[[Category: IOD]] | [[Category: IOD]] | ||
[[Category: antiterminator]] | [[Category: antiterminator]] | ||
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[[Category: two components system]] | [[Category: two components system]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:00:15 2008'' |
Revision as of 13:00, 21 February 2008
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Crystal structure of Rv1626
Overview
We describe the crystal structure of Rv1626 from Mycobacterium tuberculosis at 1.48 A resolution and the corresponding solution structure determined from small angle X-ray scattering. The N-terminal domain shows structural homology to the receiver domains found in bacterial two-component systems. The C-terminal domain has high structural homology to a recently discovered RNA binding domain involved in transcriptional antitermination. The molecule in solution was found to be monomeric as it is in the crystal, but in solution it undergoes a conformational change that is triggered by changes in ionic strength. This is the first structure that links the phosphorylation cascade of the two-component systems with the antitermination event in the transcriptional machinery. Rv1626 belongs to a family of proteins, which we propose calling phosphorylation-dependent transcriptional antitermination regulators, so far only found in bacteria, and includes NasT, a protein from the assimilatory nitrate/nitrite reductase operon of Azetobacter vinelandii.
About this Structure
1SD5 is a Single protein structure of sequence from Mycobacterium tuberculosis h37rv with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal and solution structure of a putative transcriptional antiterminator from Mycobacterium tuberculosis., Morth JP, Feng V, Perry LJ, Svergun DI, Tucker PA, Structure. 2004 Sep;12(9):1595-605. PMID:15341725
Page seeded by OCA on Thu Feb 21 15:00:15 2008
Categories: Mycobacterium tuberculosis h37rv | Single protein | Feng, V. | Morth, J P. | Perry, L J. | Svergun, D I. | TBSGC, TB Structural Genomics Consortium. | Tucker, P A. | IOD | Antiterminator | Protein structure initiative | Psi | Rv1626 | Structural genomics | Tb structural genomics consortium | Tbsgc | Transcriptional | Two components system
