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1skj
From Proteopedia
(New page: 200px<br /><applet load="1skj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1skj, resolution 2.0Å" /> '''COCRYSTAL STRUCTURE O...) |
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| - | [[Image:1skj.gif|left|200px]]<br /><applet load="1skj" size=" | + | [[Image:1skj.gif|left|200px]]<br /><applet load="1skj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1skj, resolution 2.0Å" /> | caption="1skj, resolution 2.0Å" /> | ||
'''COCRYSTAL STRUCTURE OF UREA-SUBSTITUTED PHOSPHOPEPTIDE COMPLEX'''<br /> | '''COCRYSTAL STRUCTURE OF UREA-SUBSTITUTED PHOSPHOPEPTIDE COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The specific association of an SH2 domain with a phosphotyrosine | + | The specific association of an SH2 domain with a phosphotyrosine (pTyr)-containing sequence of another protein precipitates a cascade of intracellular molecular interactions (signals) which effect a wide range of intracellular processes. The nonreceptor tyrosine kinase Src, which has been associated with breast cancer and osteoporosis, contains an SH2 domain. Inhibition of Src SH2-phosphoprotein interactions by small molecules will aid biological proof-of-concept studies which may lead to the development of novel therapeutic agents. Structure-based design efforts have focused on reducing the size and charge of Src SH2 ligands while increasing their ability to penetrate cells and reach the intracellular Src SH2 domain target. In this report we describe the synthesis, binding affinity, and Src SH2 cocrystal structure of a small, novel, nonpeptide, urea-containing SH2 domain ligand. |
==About this Structure== | ==About this Structure== | ||
| - | 1SKJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus] with UR2 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http:// | + | 1SKJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus] with <scene name='pdbligand=UR2:'>UR2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKJ OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
| - | [[Category: Holland, D | + | [[Category: Holland, D R.]] |
| - | [[Category: Rubin, J | + | [[Category: Rubin, J R.]] |
[[Category: UR2]] | [[Category: UR2]] | ||
[[Category: peptidomimetic]] | [[Category: peptidomimetic]] | ||
| Line 24: | Line 24: | ||
[[Category: v-src sh2 domain]] | [[Category: v-src sh2 domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:29 2008'' |
Revision as of 13:02, 21 February 2008
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COCRYSTAL STRUCTURE OF UREA-SUBSTITUTED PHOSPHOPEPTIDE COMPLEX
Overview
The specific association of an SH2 domain with a phosphotyrosine (pTyr)-containing sequence of another protein precipitates a cascade of intracellular molecular interactions (signals) which effect a wide range of intracellular processes. The nonreceptor tyrosine kinase Src, which has been associated with breast cancer and osteoporosis, contains an SH2 domain. Inhibition of Src SH2-phosphoprotein interactions by small molecules will aid biological proof-of-concept studies which may lead to the development of novel therapeutic agents. Structure-based design efforts have focused on reducing the size and charge of Src SH2 ligands while increasing their ability to penetrate cells and reach the intracellular Src SH2 domain target. In this report we describe the synthesis, binding affinity, and Src SH2 cocrystal structure of a small, novel, nonpeptide, urea-containing SH2 domain ligand.
About this Structure
1SKJ is a Single protein structure of sequence from Rous sarcoma virus with as ligand. Active as Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 Full crystallographic information is available from OCA.
Reference
Design, synthesis, and cocrystal structure of a nonpeptide Src SH2 domain ligand., Plummer MS, Holland DR, Shahripour A, Lunney EA, Fergus JH, Marks JS, McConnell P, Mueller WT, Sawyer TK, J Med Chem. 1997 Nov 7;40(23):3719-25. PMID:9371236
Page seeded by OCA on Thu Feb 21 15:02:29 2008
