This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Sandbox Reserved 715
From Proteopedia
| Line 8: | Line 8: | ||
===Introduction=== | ===Introduction=== | ||
| - | The | + | The β-fructosidase (β-D-fructofuranosidase EC 3. 2. 1. 26.) of the thermophilic baterium ''Thermotoga maritima'' is an enzyme which hydrolyzes sucrose to release fructose and glucose. [[Image:Invertase.gif]] |
| - | This enzyme achieves the hydrolysis with a retention molecular mechanism. Actually, the invertase hydrolyses the D-sucrose to form the D-fructose and the D-glucose. So, the substrate anomeric configuration is conserved in products. | + | This enzyme achieves the hydrolysis with a retention molecular mechanism. Actually, the invertase hydrolyses the D-sucrose to form the D-fructose and the D-glucose. So, the substrate anomeric configuration is conserved in products. The β-fructosidase belongs to the GH32 (Glycoside hydrolysases 32) according to the sequence-based classification of glycoside hydrolysases. |
| + | |||
| + | ===The GH32 family=== | ||
| + | |||
| + | The GH32 family includes over 370 members from plants, fungi and bacteria. This family contains not only invertases but other fructofuranosidases like insulinase, transfructosidase or inulinase. The glycoside hydrolases (GH) constitute a widespread enzyme group presenting a huge variety of protein folds and substrate specificities. | ||
Revision as of 22:22, 30 December 2012
Please, do not delete or modify any text or image. Thank you.
Thermotoga maritima Beta-fructosidase (invertase)
Introduction
The β-fructosidase (β-D-fructofuranosidase EC 3. 2. 1. 26.) of the thermophilic baterium Thermotoga maritima is an enzyme which hydrolyzes sucrose to release fructose and glucose. 
This enzyme achieves the hydrolysis with a retention molecular mechanism. Actually, the invertase hydrolyses the D-sucrose to form the D-fructose and the D-glucose. So, the substrate anomeric configuration is conserved in products. The β-fructosidase belongs to the GH32 (Glycoside hydrolysases 32) according to the sequence-based classification of glycoside hydrolysases.
The GH32 family
The GH32 family includes over 370 members from plants, fungi and bacteria. This family contains not only invertases but other fructofuranosidases like insulinase, transfructosidase or inulinase. The glycoside hydrolases (GH) constitute a widespread enzyme group presenting a huge variety of protein folds and substrate specificities.
|
