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1esf

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[[Category: staphylococcal enterotoxin type a]]
[[Category: staphylococcal enterotoxin type a]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:48:18 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:11:13 2007''

Revision as of 13:06, 30 October 2007


1esf, resolution 1.9Å

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STAPHYLOCOCCAL ENTEROTOXIN A

Overview

Staphylococcal enterotoxins are prototype superantigens characterized by, their ability to bind to major histocompatibility complex (MHC) class II, molecules and subsequently activate a large fraction of T-lymphocytes. The, crystal structure of staphylococcal enterotoxin type A (SEA), a 27 kDa, monomeric protein, was determined to 1.9 A resolution with an R-factor of, 19.9% by multiple isomorphous replacement. SEA is a two domain protein, composed of a beta-barrel and a beta-grasp motif demonstrating the same, general structure as staphylococcal enterotoxins SEB and TSST-1. Unique, for SEA, however, is a Zn2+ coordination site involved in MHC class II, binding. Four amino acids including Ser1, His187, His225 and Asp227 were, found to be involved in direct coordination of the metal ion. ... [(full description)]

About this Structure

1ESF is a [Single protein] structure of sequence from [Staphylococcus aureus] with CD as [ligand]. Structure known Active Sites: CD1 and CD2. Full crystallographic information is available from [OCA].

Reference

Crystal structure of the superantigen staphylococcal enterotoxin type A., Schad EM, Zaitseva I, Zaitsev VN, Dohlsten M, Kalland T, Schlievert PM, Ohlendorf DH, Svensson LA, EMBO J. 1995 Jul 17;14(14):3292-301. PMID:7628431

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