1tqh
From Proteopedia
(New page: 200px<br /><applet load="1tqh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tqh, resolution 1.63Å" /> '''Covalent Reaction in...) |
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| - | [[Image:1tqh.jpg|left|200px]]<br /><applet load="1tqh" size=" | + | [[Image:1tqh.jpg|left|200px]]<br /><applet load="1tqh" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tqh, resolution 1.63Å" /> | caption="1tqh, resolution 1.63Å" /> | ||
'''Covalent Reaction intermediate Revealed in Crystal Structure of the Geobacillus stearothermophilus Carboxylesterase Est30'''<br /> | '''Covalent Reaction intermediate Revealed in Crystal Structure of the Geobacillus stearothermophilus Carboxylesterase Est30'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Est30 is a thermophilic carboxylesterase cloned from Geobacillus | + | Est30 is a thermophilic carboxylesterase cloned from Geobacillus stearothermophilus that showed optimal hydrolysis of esters with short acyl chains at 70 degrees C. Est30 is a member of a new family of carboxylesterases with representatives in other Gram-positive bacteria. The crystal structure has been determined at 1.63A resolution using multiple anomalous dispersion data. The two-domain crystal structure showed a large domain with a modified alpha/beta hydrolase core including a seven, rather than an eight-stranded beta sheet, and a smaller cap domain comprising three alpha helices. The catalytic triad consists of residues Ser94, Asp193, and His223. A 100Da tetrahedral ligand was observed to be covalently bound to the side-chain of Ser94. The propyl acetate ligand represents the first tetrahedral intermediate in the reaction mechanism. Therefore, this Est30 crystal structure will help understand the mode of action of all enzymes in the serine hydrolase superfamily. |
==About this Structure== | ==About this Structure== | ||
| - | 1TQH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with SO4 and 4PA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] Full crystallographic information is available from [http:// | + | 1TQH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=4PA:'>4PA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TQH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Abdelal, A | + | [[Category: Abdelal, A T.]] |
| - | [[Category: Ewis, H | + | [[Category: Ewis, H E.]] |
| - | [[Category: Harrison, R | + | [[Category: Harrison, R W.]] |
[[Category: Liu, P.]] | [[Category: Liu, P.]] | ||
| - | [[Category: Lu, C | + | [[Category: Lu, C D.]] |
| - | [[Category: Wang, Y | + | [[Category: Wang, Y F.]] |
| - | [[Category: Weber, I | + | [[Category: Weber, I T.]] |
[[Category: 4PA]] | [[Category: 4PA]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: tetrahedral intermediate]] | [[Category: tetrahedral intermediate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:16:17 2008'' |
Revision as of 13:16, 21 February 2008
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Covalent Reaction intermediate Revealed in Crystal Structure of the Geobacillus stearothermophilus Carboxylesterase Est30
Overview
Est30 is a thermophilic carboxylesterase cloned from Geobacillus stearothermophilus that showed optimal hydrolysis of esters with short acyl chains at 70 degrees C. Est30 is a member of a new family of carboxylesterases with representatives in other Gram-positive bacteria. The crystal structure has been determined at 1.63A resolution using multiple anomalous dispersion data. The two-domain crystal structure showed a large domain with a modified alpha/beta hydrolase core including a seven, rather than an eight-stranded beta sheet, and a smaller cap domain comprising three alpha helices. The catalytic triad consists of residues Ser94, Asp193, and His223. A 100Da tetrahedral ligand was observed to be covalently bound to the side-chain of Ser94. The propyl acetate ligand represents the first tetrahedral intermediate in the reaction mechanism. Therefore, this Est30 crystal structure will help understand the mode of action of all enzymes in the serine hydrolase superfamily.
About this Structure
1TQH is a Single protein structure of sequence from Geobacillus stearothermophilus with and as ligands. Active as Carboxylesterase, with EC number 3.1.1.1 Full crystallographic information is available from OCA.
Reference
Covalent reaction intermediate revealed in crystal structure of the Geobacillus stearothermophilus carboxylesterase Est30., Liu P, Wang YF, Ewis HE, Abdelal AT, Lu CD, Harrison RW, Weber IT, J Mol Biol. 2004 Sep 10;342(2):551-61. PMID:15327954
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