1u3e
From Proteopedia
(New page: 200px<br /><applet load="1u3e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u3e, resolution 2.92Å" /> '''DNA binding and clea...) |
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| - | [[Image:1u3e.gif|left|200px]]<br /><applet load="1u3e" size=" | + | [[Image:1u3e.gif|left|200px]]<br /><applet load="1u3e" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u3e, resolution 2.92Å" /> | caption="1u3e, resolution 2.92Å" /> | ||
'''DNA binding and cleavage by the HNH homing endonuclease I-HmuI'''<br /> | '''DNA binding and cleavage by the HNH homing endonuclease I-HmuI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of I-HmuI, which represents the last family of homing | + | The structure of I-HmuI, which represents the last family of homing endonucleases without a defining crystallographic structure, has been determined in complex with its DNA target. A series of diverse protein structural domains and motifs, contacting sequential stretches of nucleotide bases, are distributed along the DNA target. I-HmuI contains an N-terminal domain with a DNA-binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease. The combination and exchange of these features between protein families indicates that the genetic mobility associated with homing endonucleases extends to the level of independent structural domains. I-HmuI provides an unambiguous structural connection between the His-Cys box endonucleases and the bacterial colicins, supporting the hypothesis that these enzymes diverged from a common ancestral nuclease. |
==About this Structure== | ==About this Structure== | ||
| - | 1U3E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_sp6 Enterobacteria phage sp6] with MN, SR, EDO and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1U3E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_sp6 Enterobacteria phage sp6] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=SR:'>SR</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3E OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Landthaler, M.]] | [[Category: Landthaler, M.]] | ||
| - | [[Category: Shen, B | + | [[Category: Shen, B W.]] |
| - | [[Category: Shub, D | + | [[Category: Shub, D A.]] |
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: EDO]] | [[Category: EDO]] | ||
[[Category: MN]] | [[Category: MN]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:20:14 2008'' |
Revision as of 13:20, 21 February 2008
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DNA binding and cleavage by the HNH homing endonuclease I-HmuI
Overview
The structure of I-HmuI, which represents the last family of homing endonucleases without a defining crystallographic structure, has been determined in complex with its DNA target. A series of diverse protein structural domains and motifs, contacting sequential stretches of nucleotide bases, are distributed along the DNA target. I-HmuI contains an N-terminal domain with a DNA-binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease. The combination and exchange of these features between protein families indicates that the genetic mobility associated with homing endonucleases extends to the level of independent structural domains. I-HmuI provides an unambiguous structural connection between the His-Cys box endonucleases and the bacterial colicins, supporting the hypothesis that these enzymes diverged from a common ancestral nuclease.
About this Structure
1U3E is a Single protein structure of sequence from Enterobacteria phage sp6 with , , and as ligands. Full crystallographic information is available from OCA.
Reference
DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:15313606
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