This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1u8z
From Proteopedia
(New page: 200px<br /><applet load="1u8z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u8z, resolution 1.50Å" /> '''Crystal structures o...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1u8z.jpg|left|200px]]<br /><applet load="1u8z" size=" | + | [[Image:1u8z.jpg|left|200px]]<br /><applet load="1u8z" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1u8z, resolution 1.50Å" /> | caption="1u8z, resolution 1.50Å" /> | ||
'''Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap'''<br /> | '''Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap'''<br /> | ||
==Overview== | ==Overview== | ||
| - | RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst | + | RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst complex and RalBP1, a GAP for Rho proteins. We report the crystal structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules in the asymmetric unit of the Ral-GppNHp structure. In the other molecule in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp gamma-phosphate in an environment in which switch I is pulled away from the nucleotide and switch II is found in a tight beta turn. Clustering of conserved residues on the surface of Ral-GppNHp identifies two putative sites for protein-protein interaction. One site is adjacent to switch I. The other is modulated by switch II and is obstructed in Ral-GDP. The Ral structures are discussed in the context of the published structures of the Ral/Sec5 complex, Ras, and Rap. |
==About this Structure== | ==About this Structure== | ||
| - | 1U8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saguinus_oedipus Saguinus oedipus] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1U8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saguinus_oedipus Saguinus oedipus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8Z OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Kosak, J.]] | [[Category: Kosak, J.]] | ||
[[Category: Mattos, C.]] | [[Category: Mattos, C.]] | ||
| - | [[Category: Nicely, N | + | [[Category: Nicely, N I.]] |
| - | [[Category: Serrano, V | + | [[Category: Serrano, V de.]] |
[[Category: GDP]] | [[Category: GDP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 28: | Line 28: | ||
[[Category: ras]] | [[Category: ras]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:50 2008'' |
Revision as of 13:21, 21 February 2008
|
Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap
Overview
RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst complex and RalBP1, a GAP for Rho proteins. We report the crystal structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules in the asymmetric unit of the Ral-GppNHp structure. In the other molecule in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp gamma-phosphate in an environment in which switch I is pulled away from the nucleotide and switch II is found in a tight beta turn. Clustering of conserved residues on the surface of Ral-GppNHp identifies two putative sites for protein-protein interaction. One site is adjacent to switch I. The other is modulated by switch II and is obstructed in Ral-GDP. The Ral structures are discussed in the context of the published structures of the Ral/Sec5 complex, Ras, and Rap.
About this Structure
1U8Z is a Single protein structure of sequence from Saguinus oedipus with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:15530367
Page seeded by OCA on Thu Feb 21 15:21:50 2008
Categories: Saguinus oedipus | Single protein | Kosak, J. | Mattos, C. | Nicely, N I. | Serrano, V de. | GDP | MG | Gdp | Gmppnp | Gnp | Gppnhp | Gtp | Gtpase | Ral | Ras
