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1ucb

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(New page: 200px<br /> <applet load="1ucb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ucb, resolution 2.5&Aring;" /> '''STRUCTURE OF UNCOMPL...)
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<applet load="1ucb" size="450" color="white" frame="true" align="right" spinBox="true"
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caption="1ucb, resolution 2.5&Aring;" />
caption="1ucb, resolution 2.5&Aring;" />
'''STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)'''<br />
'''STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)'''<br />
==Overview==
==Overview==
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The X-ray structure of the uncomplexed human chimeric Fab' of the, anti-tumor antibody BR96 has been determined at 2.6 A resolution. The, structure has been compared with Lewis Y antigen-complexed structures of, BR96 which were determined previously. The comparison reveals segmental, motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite, its significant main-chain contacts to the carbohydrate antigen. In light, of the uncomplexed chimeric Fab' structure reported here, the previously, observed high mobility of the CL:CH1 domains of the complexed chimeric, BR96 Fab is rationalized as a "swinging" motion approximately about the, axis of the elbow bend.
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The X-ray structure of the uncomplexed human chimeric Fab' of the anti-tumor antibody BR96 has been determined at 2.6 A resolution. The structure has been compared with Lewis Y antigen-complexed structures of BR96 which were determined previously. The comparison reveals segmental motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite its significant main-chain contacts to the carbohydrate antigen. In light of the uncomplexed chimeric Fab' structure reported here, the previously observed high mobility of the CL:CH1 domains of the complexed chimeric BR96 Fab is rationalized as a "swinging" motion approximately about the axis of the elbow bend.
==Disease==
==Disease==
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==About this Structure==
==About this Structure==
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1UCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UCB OCA].
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1UCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCB OCA].
==Reference==
==Reference==
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[[Category: immunoglobulin]]
[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:33:14 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:22:54 2008''

Revision as of 13:23, 21 February 2008

Image:1ucb.gif

1ucb, resolution 2.5Å

Drag the structure with the mouse to rotate

STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)

Contents

Overview

The X-ray structure of the uncomplexed human chimeric Fab' of the anti-tumor antibody BR96 has been determined at 2.6 A resolution. The structure has been compared with Lewis Y antigen-complexed structures of BR96 which were determined previously. The comparison reveals segmental motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite its significant main-chain contacts to the carbohydrate antigen. In light of the uncomplexed chimeric Fab' structure reported here, the previously observed high mobility of the CL:CH1 domains of the complexed chimeric BR96 Fab is rationalized as a "swinging" motion approximately about the axis of the elbow bend.

Disease

Known disease associated with this structure: Kappa light chain deficiency OMIM:[147200]

About this Structure

1UCB is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.

Reference

X-ray structure of the uncomplexed anti-tumor antibody BR96 and comparison with its antigen-bound form., Sheriff S, Chang CY, Jeffrey PD, Bajorath J, J Mol Biol. 1996 Jun 28;259(5):938-46. PMID:8683596

Page seeded by OCA on Thu Feb 21 15:22:54 2008

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