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1ul2
From Proteopedia
(New page: 200px<br /><applet load="1ul2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ul2" /> '''Solution Conformation of alpha-Conotoxin GIC...) |
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| - | [[Image:1ul2.jpg|left|200px]]<br /><applet load="1ul2" size=" | + | [[Image:1ul2.jpg|left|200px]]<br /><applet load="1ul2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ul2" /> | caption="1ul2" /> | ||
'''Solution Conformation of alpha-Conotoxin GIC'''<br /> | '''Solution Conformation of alpha-Conotoxin GIC'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Alpha-conotoxin GIC is a 16-residue peptide isolated from the venom of the | + | Alpha-conotoxin GIC is a 16-residue peptide isolated from the venom of the cone snail Conus geographus. Alpha-conotoxin GIC potently blocks the alpha3beta2 subtype of human nicotinic acetylcholine receptor, showing a high selectivity for neuronal versus muscle subtype [McIntosh, Dowell, Watkins, Garrett, Yoshikami, and Olivera (2002) J. Biol. Chem. 277, 33610-33615]. We have now determined the three-dimensional solution structure of alpha-conotoxin GIC by NMR spectroscopy. The structure of alpha-conotoxin GIC is well defined with backbone and heavy atom root mean square deviations (residues 2-16) of 0.53 A and 0.96 A respectively. Structure and surface comparison of alpha-conotoxin GIC with the other alpha4/7 subfamily conotoxins reveals unique structural aspects of alpha-conotoxin GIC. In particular, the structural comparison between alpha-conotoxins GIC and MII indicates molecular features that may confer their similar receptor specificity profile, as well as those that provide the unique binding characteristics of alpha-conotoxin GIC. |
==About this Structure== | ==About this Structure== | ||
| - | 1UL2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1UL2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UL2 OCA]. |
==Reference== | ==Reference== | ||
Solution conformation of alpha-conotoxin GIC, a novel potent antagonist of alpha3beta2 nicotinic acetylcholine receptors., Chi SW, Kim DH, Olivera BM, McIntosh JM, Han KH, Biochem J. 2004 Jun 1;380(Pt 2):347-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14992691 14992691] | Solution conformation of alpha-conotoxin GIC, a novel potent antagonist of alpha3beta2 nicotinic acetylcholine receptors., Chi SW, Kim DH, Olivera BM, McIntosh JM, Han KH, Biochem J. 2004 Jun 1;380(Pt 2):347-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14992691 14992691] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chi, S | + | [[Category: Chi, S W.]] |
| - | [[Category: Han, K | + | [[Category: Han, K H.]] |
| - | [[Category: Kim, D | + | [[Category: Kim, D H.]] |
| - | [[Category: McIntosh, J | + | [[Category: McIntosh, J M.]] |
| - | [[Category: Olivera, B | + | [[Category: Olivera, B M.]] |
[[Category: NH2]] | [[Category: NH2]] | ||
[[Category: alpha-helix]] | [[Category: alpha-helix]] | ||
| Line 23: | Line 23: | ||
[[Category: two disulfide bonds]] | [[Category: two disulfide bonds]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:42 2008'' |
Revision as of 13:25, 21 February 2008
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Solution Conformation of alpha-Conotoxin GIC
Overview
Alpha-conotoxin GIC is a 16-residue peptide isolated from the venom of the cone snail Conus geographus. Alpha-conotoxin GIC potently blocks the alpha3beta2 subtype of human nicotinic acetylcholine receptor, showing a high selectivity for neuronal versus muscle subtype [McIntosh, Dowell, Watkins, Garrett, Yoshikami, and Olivera (2002) J. Biol. Chem. 277, 33610-33615]. We have now determined the three-dimensional solution structure of alpha-conotoxin GIC by NMR spectroscopy. The structure of alpha-conotoxin GIC is well defined with backbone and heavy atom root mean square deviations (residues 2-16) of 0.53 A and 0.96 A respectively. Structure and surface comparison of alpha-conotoxin GIC with the other alpha4/7 subfamily conotoxins reveals unique structural aspects of alpha-conotoxin GIC. In particular, the structural comparison between alpha-conotoxins GIC and MII indicates molecular features that may confer their similar receptor specificity profile, as well as those that provide the unique binding characteristics of alpha-conotoxin GIC.
About this Structure
1UL2 is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Solution conformation of alpha-conotoxin GIC, a novel potent antagonist of alpha3beta2 nicotinic acetylcholine receptors., Chi SW, Kim DH, Olivera BM, McIntosh JM, Han KH, Biochem J. 2004 Jun 1;380(Pt 2):347-52. PMID:14992691
Page seeded by OCA on Thu Feb 21 15:25:42 2008
