1v4t
From Proteopedia
(New page: 200px<br /> <applet load="1v4t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v4t, resolution 3.40Å" /> '''Crystal structure o...) |
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caption="1v4t, resolution 3.40Å" /> | caption="1v4t, resolution 3.40Å" /> | ||
'''Crystal structure of human glucokinase'''<br /> | '''Crystal structure of human glucokinase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Glucokinase is a monomeric enzyme that displays a low affinity for glucose | + | Glucokinase is a monomeric enzyme that displays a low affinity for glucose and a sigmoidal saturation curve for its substrate, two properties that are important for its playing the role of a glucose sensor in pancreas and liver. The molecular basis for these two properties is not well understood. Herein we report the crystal structures of glucokinase in its active and inactive forms, which demonstrate that global conformational change, including domain reorganization, is induced by glucose binding. This suggests that the positive cooperativity of monomeric glucokinase obeys the "mnemonical mechanism" rather than the well-known concerted model. These structures also revealed an allosteric site through which small molecules may modulate the kinetic properties of the enzyme. This finding provided the mechanistic basis for activation of glucokinase as a potential therapeutic approach for treating type 2 diabetes mellitus. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1V4T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hexokinase Hexokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1] Full crystallographic information is available from [http:// | + | 1V4T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hexokinase Hexokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V4T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hexokinase iv]] | [[Category: hexokinase iv]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:31:29 2008'' |
Revision as of 13:31, 21 February 2008
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Crystal structure of human glucokinase
Contents |
Overview
Glucokinase is a monomeric enzyme that displays a low affinity for glucose and a sigmoidal saturation curve for its substrate, two properties that are important for its playing the role of a glucose sensor in pancreas and liver. The molecular basis for these two properties is not well understood. Herein we report the crystal structures of glucokinase in its active and inactive forms, which demonstrate that global conformational change, including domain reorganization, is induced by glucose binding. This suggests that the positive cooperativity of monomeric glucokinase obeys the "mnemonical mechanism" rather than the well-known concerted model. These structures also revealed an allosteric site through which small molecules may modulate the kinetic properties of the enzyme. This finding provided the mechanistic basis for activation of glucokinase as a potential therapeutic approach for treating type 2 diabetes mellitus.
Disease
Known diseases associated with this structure: Diabetes mellitus, gestational OMIM:[138079], Diabetes mellitus, noninsulin-dependent, late onset OMIM:[138079], Diabetes mellitus, permanent neonatal OMIM:[138079], Hyperinsulinemic hypoglycemia, familial, 3 OMIM:[138079], MODY, type II OMIM:[138079]
About this Structure
1V4T is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Hexokinase, with EC number 2.7.1.1 Full crystallographic information is available from OCA.
Reference
Structural basis for allosteric regulation of the monomeric allosteric enzyme human glucokinase., Kamata K, Mitsuya M, Nishimura T, Eiki J, Nagata Y, Structure. 2004 Mar;12(3):429-38. PMID:15016359
Page seeded by OCA on Thu Feb 21 15:31:29 2008
Categories: Hexokinase | Homo sapiens | Single protein | Eiki, J. | Kamata, K. | Mitsuya, M. | Nagata, Y. | Nishimura, T. | NA | SO4 | Allosteric enzyme | Diabetes | Hexokinase iv
