1v7b
From Proteopedia
(New page: 200px<br /><applet load="1v7b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v7b, resolution 1.9Å" /> '''TetR-family transcrip...) |
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| - | [[Image:1v7b.gif|left|200px]]<br /><applet load="1v7b" size=" | + | [[Image:1v7b.gif|left|200px]]<br /><applet load="1v7b" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1v7b, resolution 1.9Å" /> | caption="1v7b, resolution 1.9Å" /> | ||
'''TetR-family transcription factor CGL2612 from C.glutamicum'''<br /> | '''TetR-family transcription factor CGL2612 from C.glutamicum'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The emergence of antibiotic-resistant bacteria often causes serious | + | The emergence of antibiotic-resistant bacteria often causes serious clinical problems. The TetR family is one of the major transcription factor families that regulate expression of genes involved in bacterial antimicrobial resistance systems. CGL2612 protein is a transcription factor newly identified by genomic DNA analysis on Corynebacterium glutamicum, which belongs to the mycolic acid-containing Actinomycetales, including the well known pathogens Corynebacterium diphtheriae and Mycobacterium tuberculosis. Crystal structure analysis showed that the CGL2612 protein exhibits significant structural similarity to the multidrug resistance (MDR)-related transcription factor QacR from Staphylococcus aureus, despite poor amino acid sequence similarity between these proteins. Binding DNA sequence analysis of CGL2612 protein using the systematic evolution of ligands by the exponential enrichment (systematic evolution of ligands by exponential enrichment, or SELEX) method revealed that this protein is a new member of the TetR family, which regulates expression of the immediately upstream gene, cgl2611, probably encoding a major facilitator superfamily permease. Subsequent functional analyses confirmed a function of the CGL2612 as a transcriptional repressor responsible for the antimicrobial resistance system in C. glutamicum. The strategy used in the present study is one of the most convenient and powerful methods to analyze functionally unknown transcription factors, and the results obtained here will contribute to our understanding of the drug resistance mechanism not only in C. glutamicum but also in the related bacteria, C. diphtheriae and M. tuberculosis. |
==About this Structure== | ==About this Structure== | ||
| - | 1V7B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum_atcc_13032 Corynebacterium glutamicum atcc 13032]. Full crystallographic information is available from [http:// | + | 1V7B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum_atcc_13032 Corynebacterium glutamicum atcc 13032]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V7B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hth motif]] | [[Category: hth motif]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:32:18 2008'' |
Revision as of 13:32, 21 February 2008
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TetR-family transcription factor CGL2612 from C.glutamicum
Overview
The emergence of antibiotic-resistant bacteria often causes serious clinical problems. The TetR family is one of the major transcription factor families that regulate expression of genes involved in bacterial antimicrobial resistance systems. CGL2612 protein is a transcription factor newly identified by genomic DNA analysis on Corynebacterium glutamicum, which belongs to the mycolic acid-containing Actinomycetales, including the well known pathogens Corynebacterium diphtheriae and Mycobacterium tuberculosis. Crystal structure analysis showed that the CGL2612 protein exhibits significant structural similarity to the multidrug resistance (MDR)-related transcription factor QacR from Staphylococcus aureus, despite poor amino acid sequence similarity between these proteins. Binding DNA sequence analysis of CGL2612 protein using the systematic evolution of ligands by the exponential enrichment (systematic evolution of ligands by exponential enrichment, or SELEX) method revealed that this protein is a new member of the TetR family, which regulates expression of the immediately upstream gene, cgl2611, probably encoding a major facilitator superfamily permease. Subsequent functional analyses confirmed a function of the CGL2612 as a transcriptional repressor responsible for the antimicrobial resistance system in C. glutamicum. The strategy used in the present study is one of the most convenient and powerful methods to analyze functionally unknown transcription factors, and the results obtained here will contribute to our understanding of the drug resistance mechanism not only in C. glutamicum but also in the related bacteria, C. diphtheriae and M. tuberculosis.
About this Structure
1V7B is a Single protein structure of sequence from Corynebacterium glutamicum atcc 13032. Full crystallographic information is available from OCA.
Reference
The CGL2612 protein from Corynebacterium glutamicum is a drug resistance-related transcriptional repressor: structural and functional analysis of a newly identified transcription factor from genomic DNA analysis., Itou H, Okada U, Suzuki H, Yao M, Wachi M, Watanabe N, Tanaka I, J Biol Chem. 2005 Nov 18;280(46):38711-9. Epub 2005 Sep 15. PMID:16166084
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