1vck
From Proteopedia
(New page: 200px<br /><applet load="1vck" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vck, resolution 1.90Å" /> '''Crystal structure of...) |
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| - | [[Image:1vck.gif|left|200px]]<br /><applet load="1vck" size=" | + | [[Image:1vck.gif|left|200px]]<br /><applet load="1vck" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vck, resolution 1.90Å" /> | caption="1vck, resolution 1.90Å" /> | ||
'''Crystal structure of ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10'''<br /> | '''Crystal structure of ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The carbazole 1,9a-dioxygenase (CARDO) system of Pseudomonas resinovorans | + | The carbazole 1,9a-dioxygenase (CARDO) system of Pseudomonas resinovorans strain CA10 catalyzes the dioxygenation of carbazole; the 9aC carbon bonds to a nitrogen atom and its adjacent 1C carbon as the initial reaction in the mineralization pathway. The CARDO system is composed of ferredoxin reductase (CarAd), ferredoxin (CarAc), and terminal oxygenase (CarAa). CarAc acts as a mediator in the electron transfer from CarAd to CarAa. To understand the structural basis of the protein-protein interactions during electron transport in the CARDO system, the crystal structure of CarAc was determined at 1.9 A resolution by molecular replacement using the structure of BphF, the biphenyl 2,3-dioxygenase ferredoxin from Burkholderia cepacia strain LB400 as a search model. CarAc is composed of three beta-sheets, and the structure can be divided into two domains, a cluster-binding domain and a basal domain. The Rieske [2Fe-2S] cluster is located at the tip of the cluster-binding domain, where it is exposed to solvent. While the overall folding of CarAc and BphF is strongly conserved, the properties of their surfaces are very different from each other. The structure of the cluster-binding domain of CarAc is more compact and protruding than that of BphF, and the distribution of electric charge on its molecular surface is very different. Such differences are thought to explain why these ferredoxins can act as electron mediators in respective electron transport chains composed of different-featured components. |
==About this Structure== | ==About this Structure== | ||
| - | 1VCK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_resinovorans Pseudomonas resinovorans] with FE, FES and S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1VCK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_resinovorans Pseudomonas resinovorans] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=FES:'>FES</scene> and <scene name='pdbligand=S:'>S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VCK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kobashi, N.]] | [[Category: Kobashi, N.]] | ||
[[Category: Mizuno, H.]] | [[Category: Mizuno, H.]] | ||
| - | [[Category: Nam, J | + | [[Category: Nam, J W.]] |
[[Category: Noguchi, H.]] | [[Category: Noguchi, H.]] | ||
[[Category: Nojiri, H.]] | [[Category: Nojiri, H.]] | ||
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[[Category: rieske-type ferredoxin]] | [[Category: rieske-type ferredoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:33:49 2008'' |
Revision as of 13:33, 21 February 2008
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Crystal structure of ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10
Overview
The carbazole 1,9a-dioxygenase (CARDO) system of Pseudomonas resinovorans strain CA10 catalyzes the dioxygenation of carbazole; the 9aC carbon bonds to a nitrogen atom and its adjacent 1C carbon as the initial reaction in the mineralization pathway. The CARDO system is composed of ferredoxin reductase (CarAd), ferredoxin (CarAc), and terminal oxygenase (CarAa). CarAc acts as a mediator in the electron transfer from CarAd to CarAa. To understand the structural basis of the protein-protein interactions during electron transport in the CARDO system, the crystal structure of CarAc was determined at 1.9 A resolution by molecular replacement using the structure of BphF, the biphenyl 2,3-dioxygenase ferredoxin from Burkholderia cepacia strain LB400 as a search model. CarAc is composed of three beta-sheets, and the structure can be divided into two domains, a cluster-binding domain and a basal domain. The Rieske [2Fe-2S] cluster is located at the tip of the cluster-binding domain, where it is exposed to solvent. While the overall folding of CarAc and BphF is strongly conserved, the properties of their surfaces are very different from each other. The structure of the cluster-binding domain of CarAc is more compact and protruding than that of BphF, and the distribution of electric charge on its molecular surface is very different. Such differences are thought to explain why these ferredoxins can act as electron mediators in respective electron transport chains composed of different-featured components.
About this Structure
1VCK is a Single protein structure of sequence from Pseudomonas resinovorans with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10, a novel Rieske non-heme iron oxygenase system., Nam JW, Noguchi H, Fujimoto Z, Mizuno H, Ashikawa Y, Abo M, Fushinobu S, Kobashi N, Wakagi T, Iwata K, Yoshida T, Habe H, Yamane H, Omori T, Nojiri H, Proteins. 2005 Mar 1;58(4):779-89. PMID:15645447
Page seeded by OCA on Thu Feb 21 15:33:49 2008
Categories: Pseudomonas resinovorans | Single protein | Fujiomoto, Z. | Fushinobu, S. | Habe, H. | Iwata, K. | Kobashi, N. | Mizuno, H. | Nam, J W. | Noguchi, H. | Nojiri, H. | Omori, T. | Yamane, H. | Yoshida, T. | FE | FES | S | 9a-dioxygenase | Angular dioxygenase | Carac | Carbazole 1 | Pseudomonas resinovorans strain ca10 | Rieske non-heme iron oxygenase system | Rieske-type ferredoxin
