1vpt
From Proteopedia
(New page: 200px<br /><applet load="1vpt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vpt, resolution 1.8Å" /> '''AS11 VARIANT OF VACCI...) |
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| - | [[Image:1vpt.jpg|left|200px]]<br /><applet load="1vpt" size=" | + | [[Image:1vpt.jpg|left|200px]]<br /><applet load="1vpt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vpt, resolution 1.8Å" /> | caption="1vpt, resolution 1.8Å" /> | ||
'''AS11 VARIANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE'''<br /> | '''AS11 VARIANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | VP39 is a bifunctional vaccinia virus protein that acts as both an mRNA | + | VP39 is a bifunctional vaccinia virus protein that acts as both an mRNA cap-specific RNA 2'-O-methyltransferase and a poly(A) polymerase processivity factor. Here, we report the 1.85 A crystal structure of a VP39 variant complexed with its AdoMet cofactor. VP39 comprises a single core domain with structural similarity to the catalytic domains of other methyltransferases. Surface features and mutagenesis data suggest two possible RNA-binding sites with novel underlying architecture, one of which forms a cleft spanning the region adjacent to the methyltransferase active site. This report provides a prototypic structure for an RNA methyltransferase, a protein that interacts with the mRNA 5' cap, and an intact poxvirus protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1VPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus] with SAM as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19] Full crystallographic information is available from [http:// | + | 1VPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus] with <scene name='pdbligand=SAM:'>SAM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VPT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vaccinia virus]] | [[Category: Vaccinia virus]] | ||
| - | [[Category: Gershon, P | + | [[Category: Gershon, P D.]] |
| - | [[Category: Hodel, A | + | [[Category: Hodel, A E.]] |
| - | [[Category: Quiocho, F | + | [[Category: Quiocho, F A.]] |
[[Category: Shi, X.]] | [[Category: Shi, X.]] | ||
[[Category: SAM]] | [[Category: SAM]] | ||
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[[Category: vaccinia]] | [[Category: vaccinia]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:37:20 2008'' |
Revision as of 13:37, 21 February 2008
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AS11 VARIANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYL-L-METHIONINE
Overview
VP39 is a bifunctional vaccinia virus protein that acts as both an mRNA cap-specific RNA 2'-O-methyltransferase and a poly(A) polymerase processivity factor. Here, we report the 1.85 A crystal structure of a VP39 variant complexed with its AdoMet cofactor. VP39 comprises a single core domain with structural similarity to the catalytic domains of other methyltransferases. Surface features and mutagenesis data suggest two possible RNA-binding sites with novel underlying architecture, one of which forms a cleft spanning the region adjacent to the methyltransferase active site. This report provides a prototypic structure for an RNA methyltransferase, a protein that interacts with the mRNA 5' cap, and an intact poxvirus protein.
About this Structure
1VPT is a Single protein structure of sequence from Vaccinia virus with as ligand. Active as Polynucleotide adenylyltransferase, with EC number 2.7.7.19 Full crystallographic information is available from OCA.
Reference
The 1.85 A structure of vaccinia protein VP39: a bifunctional enzyme that participates in the modification of both mRNA ends., Hodel AE, Gershon PD, Shi X, Quiocho FA, Cell. 1996 Apr 19;85(2):247-56. PMID:8612277
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