1vs5
From Proteopedia
(New page: 200px<br /><applet load="1vs5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vs5, resolution 3.46Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1vs5.gif|left|200px]]<br /><applet load="1vs5" size=" | + | [[Image:1vs5.gif|left|200px]]<br /><applet load="1vs5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1vs5, resolution 3.46Å" /> | caption="1vs5, resolution 3.46Å" /> | ||
'''Crystal structure of the bacterial ribosome from escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400.'''<br /> | '''Crystal structure of the bacterial ribosome from escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The prokaryotic ribosome is an important target of antibiotic action. We | + | The prokaryotic ribosome is an important target of antibiotic action. We determined the X-ray structure of the aminoglycoside kasugamycin (Ksg) in complex with the Escherichia coli 70S ribosome at 3.5-A resolution. The structure reveals that the drug binds within the messenger RNA channel of the 30S subunit between the universally conserved G926 and A794 nucleotides in 16S ribosomal RNA, which are sites of Ksg resistance. To our surprise, Ksg resistance mutations do not inhibit binding of the drug to the ribosome. The present structural and biochemical results indicate that inhibition by Ksg and Ksg resistance are closely linked to the structure of the mRNA at the junction of the peptidyl-tRNA and exit-tRNA sites (P and E sites). |
==About this Structure== | ==About this Structure== | ||
| - | 1VS5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with KSG and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1VS5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=KSG:'>KSG</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VS5 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cate, J | + | [[Category: Cate, J H.D.]] |
| - | [[Category: Schuwirth, B | + | [[Category: Schuwirth, B S.]] |
[[Category: Vila-Sanjurjo, A.]] | [[Category: Vila-Sanjurjo, A.]] | ||
[[Category: KSG]] | [[Category: KSG]] | ||
| Line 21: | Line 21: | ||
[[Category: ribosome]] | [[Category: ribosome]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:38:03 2008'' |
Revision as of 13:38, 21 February 2008
|
Crystal structure of the bacterial ribosome from escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400.
Overview
The prokaryotic ribosome is an important target of antibiotic action. We determined the X-ray structure of the aminoglycoside kasugamycin (Ksg) in complex with the Escherichia coli 70S ribosome at 3.5-A resolution. The structure reveals that the drug binds within the messenger RNA channel of the 30S subunit between the universally conserved G926 and A794 nucleotides in 16S ribosomal RNA, which are sites of Ksg resistance. To our surprise, Ksg resistance mutations do not inhibit binding of the drug to the ribosome. The present structural and biochemical results indicate that inhibition by Ksg and Ksg resistance are closely linked to the structure of the mRNA at the junction of the peptidyl-tRNA and exit-tRNA sites (P and E sites).
About this Structure
1VS5 is a Protein complex structure of sequences from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.
Reference
Structural analysis of kasugamycin inhibition of translation., Schuwirth BS, Day JM, Hau CW, Janssen GR, Dahlberg AE, Cate JH, Vila-Sanjurjo A, Nat Struct Mol Biol. 2006 Oct;13(10):879-86. Epub 2006 Sep 24. PMID:16998486
Page seeded by OCA on Thu Feb 21 15:38:03 2008
