This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1gx1
From Proteopedia
| Line 26: | Line 26: | ||
[[Category: lyase]] | [[Category: lyase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:21:55 2007'' |
Revision as of 13:17, 30 October 2007
|
STRUCTURE OF 2C-METHYL-D-ERYTHRITOL-2,4-CYCLODIPHOSPHATE SYNTHASE
Overview
The crystal structure of the zinc enzyme Escherichia coli, 2C-methyl-d-erythritol 2,4-cyclodiphosphate synthase in complex with, cytidine 5'-diphosphate and Mn(2+) has been determined to 1.8-A, resolution. This enzyme is essential in E. coli and participates in the, nonmevalonate pathway of isoprenoid biosynthesis, a critical pathway, present in some bacterial and apicomplexans but distinct from that used by, mammals. Our analysis reveals a homotrimer, built around a beta prism, carrying three active sites, each of which is formed in a cleft between, pairs of subunits. Residues from two subunits recognize and bind the, nucleotide in an active site that contains a Zn(2+) with tetrahedral, coordination. A Mn(2+), with octahedral geometry, is positioned between, the alpha and beta phosphates ... [(full description)]
About this Structure
1GX1 is a [Single protein] structure of sequence from [Escherichia coli] with ZN, MN, SO4 and CDF as [ligands]. Active as [2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase], with EC number [4.6.1.12]. Structure known Active Site: CA1. Full crystallographic information is available from [OCA].
Reference
Structure of 2C-methyl-D-erythritol 2,4- cyclodiphosphate synthase: an essential enzyme for isoprenoid biosynthesis and target for antimicrobial drug development., Kemp LE, Bond CS, Hunter WN, Proc Natl Acad Sci U S A. 2002 May 14;99(10):6591-6. Epub 2002 May 7. PMID:11997478
Page seeded by OCA on Tue Oct 30 15:21:55 2007
