1w9d
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to | + | Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to hydrolyse glucosinolates, a unique family of molecules bearing an anomeric O-sulfated thiohydroximate function. Non-hydrolysable myrosinase inhibitors have been devised and studied for their biological interaction. Diverse modifications of the O-sulfate moiety did not result in a significant inhibitory effect, whereas replacing the D-glucopyrano residue by its carba-analogue allowed inhibition to take place. X-Ray experiments carried out after soaking allowed for the first time inclusion of a non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning of the aglycon part in its pocket is being used as a guide for the development of simplified and more potent inhibitors. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Sinapis alba]] | [[Category: Sinapis alba]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Transferred entry: 3 | + | [[Category: Transferred entry: 3 2.1 147]] |
[[Category: Arzt, S.]] | [[Category: Arzt, S.]] | ||
[[Category: Bourderioux, A.]] | [[Category: Bourderioux, A.]] | ||
| - | [[Category: Burmeister, W | + | [[Category: Burmeister, W P.]] |
[[Category: Cottaz, S.]] | [[Category: Cottaz, S.]] | ||
[[Category: Gueyrard, D.]] | [[Category: Gueyrard, D.]] | ||
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[[Category: thiohydroximate]] | [[Category: thiohydroximate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:41:51 2008'' |
Revision as of 13:41, 21 February 2008
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S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE
Overview
Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to hydrolyse glucosinolates, a unique family of molecules bearing an anomeric O-sulfated thiohydroximate function. Non-hydrolysable myrosinase inhibitors have been devised and studied for their biological interaction. Diverse modifications of the O-sulfate moiety did not result in a significant inhibitory effect, whereas replacing the D-glucopyrano residue by its carba-analogue allowed inhibition to take place. X-Ray experiments carried out after soaking allowed for the first time inclusion of a non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning of the aglycon part in its pocket is being used as a guide for the development of simplified and more potent inhibitors.
About this Structure
1W9D is a Single protein structure of sequence from Sinapis alba with , , , and as ligands. Active as Transferred entry: 3.2.1.147, with EC number 3.2.3.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors., Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P, Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:15889170
Page seeded by OCA on Thu Feb 21 15:41:51 2008
Categories: Sinapis alba | Single protein | Transferred entry: 3 2.1 147 | Arzt, S. | Bourderioux, A. | Burmeister, W P. | Cottaz, S. | Gueyrard, D. | Lefoix, M. | Rollin, P. | Tatibouet, A. | GOL | NAG | SEH | SO4 | ZN | Glucotropaeolin | Glusosinolate | Hydrolase | Myrosinase | Thio-glucoside | Thioglucosidase | Thioglycosidase | Thiohydroximate
