1wc8
From Proteopedia
(New page: 200px<br /><applet load="1wc8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wc8, resolution 1.9Å" /> '''THE CRYSTAL STRUCTURE...) |
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| - | [[Image:1wc8.jpg|left|200px]]<br /><applet load="1wc8" size=" | + | [[Image:1wc8.jpg|left|200px]]<br /><applet load="1wc8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wc8, resolution 1.9Å" /> | caption="1wc8, resolution 1.9Å" /> | ||
'''THE CRYSTAL STRUCTURE OF MOUSE BET3P'''<br /> | '''THE CRYSTAL STRUCTURE OF MOUSE BET3P'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Transport protein particle (TRAPP) is a large multiprotein complex | + | Transport protein particle (TRAPP) is a large multiprotein complex involved in endoplasmic reticulum-to-Golgi and intra-Golgi traffic. TRAPP specifically and persistently resides on Golgi membranes. Neither the mechanism of the subcellular localization nor the function of any of the individual TRAPP components is known. Here, the crystal structure of mouse Bet3p (bet3), a conserved TRAPP component, reveals a dimeric structure with hydrophobic channels. The channel entrances are located on a putative membrane-interacting surface that is distinctively flat, wide and decorated with positively charged residues. Charge-inversion mutations on the flat surface of the highly conserved yeast Bet3p led to conditional lethality, incorrect localization and membrane trafficking defects. A channel-blocking mutation led to similar defects. These data delineate a molecular mechanism of Golgi-specific targeting and anchoring of Bet3p involving the charged surface and insertion of a Golgi-specific hydrophobic moiety into the channels. This essential subunit could then direct other TRAPP components to the Golgi. |
==About this Structure== | ==About this Structure== | ||
| - | 1WC8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MYR as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1WC8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MYR:'>MYR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WC8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kim, Y | + | [[Category: Kim, Y G.]] |
| - | [[Category: Oh, B | + | [[Category: Oh, B H.]] |
[[Category: Sacher, M.]] | [[Category: Sacher, M.]] | ||
[[Category: MYR]] | [[Category: MYR]] | ||
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[[Category: vesicle transport]] | [[Category: vesicle transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:45 2008'' |
Revision as of 13:42, 21 February 2008
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THE CRYSTAL STRUCTURE OF MOUSE BET3P
Overview
Transport protein particle (TRAPP) is a large multiprotein complex involved in endoplasmic reticulum-to-Golgi and intra-Golgi traffic. TRAPP specifically and persistently resides on Golgi membranes. Neither the mechanism of the subcellular localization nor the function of any of the individual TRAPP components is known. Here, the crystal structure of mouse Bet3p (bet3), a conserved TRAPP component, reveals a dimeric structure with hydrophobic channels. The channel entrances are located on a putative membrane-interacting surface that is distinctively flat, wide and decorated with positively charged residues. Charge-inversion mutations on the flat surface of the highly conserved yeast Bet3p led to conditional lethality, incorrect localization and membrane trafficking defects. A channel-blocking mutation led to similar defects. These data delineate a molecular mechanism of Golgi-specific targeting and anchoring of Bet3p involving the charged surface and insertion of a Golgi-specific hydrophobic moiety into the channels. This essential subunit could then direct other TRAPP components to the Golgi.
About this Structure
1WC8 is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of bet3 reveals a novel mechanism for Golgi localization of tethering factor TRAPP., Kim YG, Sohn EJ, Seo J, Lee KJ, Lee HS, Hwang I, Whiteway M, Sacher M, Oh BH, Nat Struct Mol Biol. 2005 Jan;12(1):38-45. Epub 2004 Dec 19. PMID:15608655
Page seeded by OCA on Thu Feb 21 15:42:45 2008
