This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1wij
From Proteopedia
(New page: 200px<br /><applet load="1wij" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wij" /> '''Solution Structure of the DNA-Binding Domain...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1wij.jpg|left|200px]]<br /><applet load="1wij" size=" | + | [[Image:1wij.jpg|left|200px]]<br /><applet load="1wij" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wij" /> | caption="1wij" /> | ||
'''Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3'''<br /> | '''Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are essential | + | Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are essential transcription factors in the ethylene signaling of higher plants. The EIN3/EIL proteins bind to the promoter regions of the downstream genes and regulate their expression. The location of the DNA-binding domain (DBD) in the primary structure was unclear, since the proteins show no sequence similarity to other known DBDs. Here, we identify the major DBD of an EIN3/EIL protein, Arabidopsis thaliana EIL3, containing a key mutational site for DNA binding and signaling (ein3-3 site), and determine its solution structure by NMR spectroscopy. The structure consists of five alpha-helices, possessing a novel fold dissimilar to known DBD structures. By a chemical-shift perturbation analysis, a region including the ein3-3 site is suggested to be involved in DNA binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1WIJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http:// | + | 1WIJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WIJ OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Inoue, M.]] | [[Category: Inoue, M.]] | ||
[[Category: Kigawa, T.]] | [[Category: Kigawa, T.]] | ||
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Yamasaki, K.]] | [[Category: Yamasaki, K.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
| Line 23: | Line 23: | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:44:44 2008'' |
Revision as of 13:44, 21 February 2008
|
Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3
Overview
Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are essential transcription factors in the ethylene signaling of higher plants. The EIN3/EIL proteins bind to the promoter regions of the downstream genes and regulate their expression. The location of the DNA-binding domain (DBD) in the primary structure was unclear, since the proteins show no sequence similarity to other known DBDs. Here, we identify the major DBD of an EIN3/EIL protein, Arabidopsis thaliana EIL3, containing a key mutational site for DNA binding and signaling (ein3-3 site), and determine its solution structure by NMR spectroscopy. The structure consists of five alpha-helices, possessing a novel fold dissimilar to known DBD structures. By a chemical-shift perturbation analysis, a region including the ein3-3 site is suggested to be involved in DNA binding.
About this Structure
1WIJ is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Solution structure of the major DNA-binding domain of Arabidopsis thaliana ethylene-insensitive3-like3., Yamasaki K, Kigawa T, Inoue M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S, J Mol Biol. 2005 Apr 29;348(2):253-64. PMID:15811366
Page seeded by OCA on Thu Feb 21 15:44:44 2008
