1ww9
From Proteopedia
(New page: 200px<br /><applet load="1ww9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ww9, resolution 1.95Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ww9.gif|left|200px]]<br /><applet load="1ww9" size=" | + | [[Image:1ww9.gif|left|200px]]<br /><applet load="1ww9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ww9, resolution 1.95Å" /> | caption="1ww9, resolution 1.95Å" /> | ||
'''Crystal structure of the terminal oxygenase component of carbazole 1,9a-dioxygenase, a non-heme iron oxygenase system catalyzing the novel angular dioxygenation for carbazole and dioxin'''<br /> | '''Crystal structure of the terminal oxygenase component of carbazole 1,9a-dioxygenase, a non-heme iron oxygenase system catalyzing the novel angular dioxygenation for carbazole and dioxin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Carbazole 1,9a-dioxygenase (CARDO) catalyzes the dihydroxylation of | + | Carbazole 1,9a-dioxygenase (CARDO) catalyzes the dihydroxylation of carbazole by angular position (C9a) carbon bonding to the imino nitrogen and its adjacent C1 carbon. This reaction is an initial degradation reaction of the carbazole degradation pathway by various bacterial strains. Only a limited number of Rieske non-heme iron oxygenase systems (ROSs) can catalyze this novel reaction, termed angular dioxygenation. Angular dioxygenation is also involved in the degradation pathways of carbazole-related compounds, dioxin, and CARDO can catalyze the angular dioxygenation for dioxin. CARDO consists of a terminal oxygenase component (CARDO-O), and the electron transport components, ferredoxin (CARDO-F) and ferredoxin reductase (CARDO-R). CARDO-O has a homotrimeric structure, and governs the substrate specificity of CARDO. Here, we have determined the crystal structure of CARDO-O of Janthinobacterium sp. strain J3 at a resolution of 1.95A. The alpha3 trimeric overall structure of the CARDO-O molecule roughly corresponds to the alpha3 partial structures of other terminal oxygenase components of ROSs that have the alpha3beta3 configuration. The CARDO-O structure is a first example of the terminal oxygenase components of ROSs that have the alpha3 configuration, and revealed the presence of the specific loops that interact with a neighboring subunit, which is proposed to be indispensable for stable alpha3 interactions without structural beta subunits. The shape of the substrate-binding pocket of CARDO-O is markedly different from those of other oxygenase components involved in naphthalene and biphenyl degradation pathways. Docking simulations suggested that carbazole binds to the substrate-binding pocket in a manner suitable for catalysis of angular dioxygenation. |
==About this Structure== | ==About this Structure== | ||
| - | 1WW9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Janthinobacterium_sp._j3 Janthinobacterium sp. j3] with FE2 and FES as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1WW9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Janthinobacterium_sp._j3 Janthinobacterium sp. j3] with <scene name='pdbligand=FE2:'>FE2</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WW9 OCA]. |
==Reference== | ==Reference== | ||
| Line 17: | Line 17: | ||
[[Category: Habe, H.]] | [[Category: Habe, H.]] | ||
[[Category: Mizuno, H.]] | [[Category: Mizuno, H.]] | ||
| - | [[Category: Nam, J | + | [[Category: Nam, J W.]] |
[[Category: Noguchi, H.]] | [[Category: Noguchi, H.]] | ||
[[Category: Nojiri, H.]] | [[Category: Nojiri, H.]] | ||
| Line 31: | Line 31: | ||
[[Category: terminal oxygenase]] | [[Category: terminal oxygenase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:48:44 2008'' |
Revision as of 13:48, 21 February 2008
|
Crystal structure of the terminal oxygenase component of carbazole 1,9a-dioxygenase, a non-heme iron oxygenase system catalyzing the novel angular dioxygenation for carbazole and dioxin
Overview
Carbazole 1,9a-dioxygenase (CARDO) catalyzes the dihydroxylation of carbazole by angular position (C9a) carbon bonding to the imino nitrogen and its adjacent C1 carbon. This reaction is an initial degradation reaction of the carbazole degradation pathway by various bacterial strains. Only a limited number of Rieske non-heme iron oxygenase systems (ROSs) can catalyze this novel reaction, termed angular dioxygenation. Angular dioxygenation is also involved in the degradation pathways of carbazole-related compounds, dioxin, and CARDO can catalyze the angular dioxygenation for dioxin. CARDO consists of a terminal oxygenase component (CARDO-O), and the electron transport components, ferredoxin (CARDO-F) and ferredoxin reductase (CARDO-R). CARDO-O has a homotrimeric structure, and governs the substrate specificity of CARDO. Here, we have determined the crystal structure of CARDO-O of Janthinobacterium sp. strain J3 at a resolution of 1.95A. The alpha3 trimeric overall structure of the CARDO-O molecule roughly corresponds to the alpha3 partial structures of other terminal oxygenase components of ROSs that have the alpha3beta3 configuration. The CARDO-O structure is a first example of the terminal oxygenase components of ROSs that have the alpha3 configuration, and revealed the presence of the specific loops that interact with a neighboring subunit, which is proposed to be indispensable for stable alpha3 interactions without structural beta subunits. The shape of the substrate-binding pocket of CARDO-O is markedly different from those of other oxygenase components involved in naphthalene and biphenyl degradation pathways. Docking simulations suggested that carbazole binds to the substrate-binding pocket in a manner suitable for catalysis of angular dioxygenation.
About this Structure
1WW9 is a Single protein structure of sequence from Janthinobacterium sp. j3 with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of the terminal oxygenase component of angular dioxygenase, carbazole 1,9a-dioxygenase., Nojiri H, Ashikawa Y, Noguchi H, Nam JW, Urata M, Fujimoto Z, Uchimura H, Terada T, Nakamura S, Shimizu K, Yoshida T, Habe H, Omori T, J Mol Biol. 2005 Aug 12;351(2):355-70. PMID:16005887
Page seeded by OCA on Thu Feb 21 15:48:44 2008
Categories: Janthinobacterium sp. j3 | Single protein | Ashikawa, Y. | Fujimoto, Z. | Habe, H. | Mizuno, H. | Nam, J W. | Noguchi, H. | Nojiri, H. | Omori, T. | Urata, M. | Yoshida, T. | FE2 | FES | 9a-dioxygenase | Angular dioxygenase | Carbazole 1 | Rieske non-heme iron oxygenase system | Terminal oxygenase
