1xvr
From Proteopedia
(New page: 200px<br /><applet load="1xvr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xvr, resolution 1.40Å" /> '''echinomycin (CGTACG)...) |
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| - | [[Image:1xvr.gif|left|200px]]<br /><applet load="1xvr" size=" | + | [[Image:1xvr.gif|left|200px]]<br /><applet load="1xvr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1xvr, resolution 1.40Å" /> | caption="1xvr, resolution 1.40Å" /> | ||
'''echinomycin (CGTACG)2 complex'''<br /> | '''echinomycin (CGTACG)2 complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the bis-intercalation complex of the depsipeptide | + | The structure of the bis-intercalation complex of the depsipeptide antibiotic echinomycin with (CGTACG)2 has been redetermined at a higher resolution (1.4 A) and new high-resolution structures (1.1-1.5 A) are reported for the complexes of echinomycin with (GCGTACGC)2 (at both low and high ionic strengths) and (ACGTACGT)2. The structures show the expected Hoogsteen pairing for the base pairs flanking the intercalating chromophores on the outside and Watson-Crick pairing for both base pairs enclosed by the echinomycin. In the octamer complexes but not the hexamer complex, the echinomycin molecule, which would possess a molecular twofold axis were it not for the thioacetal bridge, shows twofold disorder. In all the structures the stacking of the base pairs and chromophores is extended by intermolecular stacking. The structures provide more precise details of the hydrogen bonding and other interactions between the bis-intercalating antibiotics and the duplex DNA than were previously available. |
==About this Structure== | ==About this Structure== | ||
| - | 1XVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG, QUI, DSN and SER as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1XVR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=QUI:'>QUI</scene>, <scene name='pdbligand=DSN:'>DSN</scene> and <scene name='pdbligand=SER:'>SER</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVR OCA]. |
==Reference== | ==Reference== | ||
Structures of complexes between echinomycin and duplex DNA., Cuesta-Seijo JA, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):442-8. Epub 2005, Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805599 15805599] | Structures of complexes between echinomycin and duplex DNA., Cuesta-Seijo JA, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):442-8. Epub 2005, Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805599 15805599] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cuesta-Seijo, J | + | [[Category: Cuesta-Seijo, J A.]] |
| - | [[Category: Sheldrick, G | + | [[Category: Sheldrick, G M.]] |
[[Category: DSN]] | [[Category: DSN]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: hoogsteen basepairs]] | [[Category: hoogsteen basepairs]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:59:10 2008'' |
Revision as of 13:59, 21 February 2008
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echinomycin (CGTACG)2 complex
Overview
The structure of the bis-intercalation complex of the depsipeptide antibiotic echinomycin with (CGTACG)2 has been redetermined at a higher resolution (1.4 A) and new high-resolution structures (1.1-1.5 A) are reported for the complexes of echinomycin with (GCGTACGC)2 (at both low and high ionic strengths) and (ACGTACGT)2. The structures show the expected Hoogsteen pairing for the base pairs flanking the intercalating chromophores on the outside and Watson-Crick pairing for both base pairs enclosed by the echinomycin. In the octamer complexes but not the hexamer complex, the echinomycin molecule, which would possess a molecular twofold axis were it not for the thioacetal bridge, shows twofold disorder. In all the structures the stacking of the base pairs and chromophores is extended by intermolecular stacking. The structures provide more precise details of the hydrogen bonding and other interactions between the bis-intercalating antibiotics and the duplex DNA than were previously available.
About this Structure
1XVR is a Protein complex structure of sequences from [1] with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Structures of complexes between echinomycin and duplex DNA., Cuesta-Seijo JA, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):442-8. Epub 2005, Mar 24. PMID:15805599
Page seeded by OCA on Thu Feb 21 15:59:10 2008
Categories: Protein complex | Cuesta-Seijo, J A. | Sheldrick, G M. | DSN | MG | QUI | SER | Bisintercalator | Hoogsteen basepairs
