1yok
From Proteopedia
(New page: 200px<br /> <applet load="1yok" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yok, resolution 2.5Å" /> '''crystal structure of...) |
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| - | [[Image:1yok.gif|left|200px]]<br /> | + | [[Image:1yok.gif|left|200px]]<br /><applet load="1yok" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1yok" size=" | + | |
caption="1yok, resolution 2.5Å" /> | caption="1yok, resolution 2.5Å" /> | ||
'''crystal structure of human LRH-1 bound with TIF-2 peptide and phosphatidylglycerol'''<br /> | '''crystal structure of human LRH-1 bound with TIF-2 peptide and phosphatidylglycerol'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Vertebrate members of the nuclear receptor NR5A subfamily, which includes | + | Vertebrate members of the nuclear receptor NR5A subfamily, which includes steroidogenic factor 1 (SF-1) and liver receptor homolog 1 (LRH-1), regulate crucial aspects of development, endocrine homeostasis, and metabolism. Mouse LRH-1 is believed to be a ligand-independent transcription factor with a large and empty hydrophobic pocket. Here we present structural and biochemical data for three other NR5A members-mouse and human SF-1 and human LRH-1-which reveal that these receptors bind phosphatidyl inositol second messengers and that ligand binding is required for maximal activity. Evolutionary analysis of structure-function relationships across the SF-1/LRH-1 subfamily indicates that ligand binding is the ancestral state of NR5A receptors and was uniquely diminished or altered in the rodent LRH-1 lineage. We propose that phospholipids regulate gene expression by directly binding to NR5A nuclear receptors. |
==About this Structure== | ==About this Structure== | ||
| - | 1YOK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with P6L as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1YOK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=P6L:'>P6L</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YOK OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bynum, J | + | [[Category: Bynum, J M.]] |
| - | [[Category: Fletterick, R | + | [[Category: Fletterick, R J.]] |
| - | [[Category: Guy, R | + | [[Category: Guy, R K.]] |
| - | [[Category: Ingraham, H | + | [[Category: Ingraham, H A.]] |
[[Category: Juzumiene, D.]] | [[Category: Juzumiene, D.]] | ||
| - | [[Category: Krylova, I | + | [[Category: Krylova, I N.]] |
[[Category: Lebedeva, L.]] | [[Category: Lebedeva, L.]] | ||
| - | [[Category: MacKay, J | + | [[Category: MacKay, J A.]] |
[[Category: Madauss, K.]] | [[Category: Madauss, K.]] | ||
[[Category: Montana, V.]] | [[Category: Montana, V.]] | ||
[[Category: Moore, J.]] | [[Category: Moore, J.]] | ||
| - | [[Category: Sablin, E | + | [[Category: Sablin, E P.]] |
[[Category: Suzawa, M.]] | [[Category: Suzawa, M.]] | ||
| - | [[Category: Thornton, J | + | [[Category: Thornton, J W.]] |
| - | [[Category: Waitt, G | + | [[Category: Waitt, G M.]] |
| - | [[Category: Williams, J | + | [[Category: Williams, J D.]] |
| - | [[Category: Williams, S | + | [[Category: Williams, S P.]] |
| - | [[Category: Willson, T | + | [[Category: Willson, T M.]] |
| - | [[Category: Xu, R | + | [[Category: Xu, R X.]] |
[[Category: P6L]] | [[Category: P6L]] | ||
[[Category: lrh-1]] | [[Category: lrh-1]] | ||
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[[Category: tif-1]] | [[Category: tif-1]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:27 2008'' |
Revision as of 14:07, 21 February 2008
|
crystal structure of human LRH-1 bound with TIF-2 peptide and phosphatidylglycerol
Overview
Vertebrate members of the nuclear receptor NR5A subfamily, which includes steroidogenic factor 1 (SF-1) and liver receptor homolog 1 (LRH-1), regulate crucial aspects of development, endocrine homeostasis, and metabolism. Mouse LRH-1 is believed to be a ligand-independent transcription factor with a large and empty hydrophobic pocket. Here we present structural and biochemical data for three other NR5A members-mouse and human SF-1 and human LRH-1-which reveal that these receptors bind phosphatidyl inositol second messengers and that ligand binding is required for maximal activity. Evolutionary analysis of structure-function relationships across the SF-1/LRH-1 subfamily indicates that ligand binding is the ancestral state of NR5A receptors and was uniquely diminished or altered in the rodent LRH-1 lineage. We propose that phospholipids regulate gene expression by directly binding to NR5A nuclear receptors.
About this Structure
1YOK is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structural analyses reveal phosphatidyl inositols as ligands for the NR5 orphan receptors SF-1 and LRH-1., Krylova IN, Sablin EP, Moore J, Xu RX, Waitt GM, MacKay JA, Juzumiene D, Bynum JM, Madauss K, Montana V, Lebedeva L, Suzawa M, Williams JD, Williams SP, Guy RK, Thornton JW, Fletterick RJ, Willson TM, Ingraham HA, Cell. 2005 Feb 11;120(3):343-55. PMID:15707893
Page seeded by OCA on Thu Feb 21 16:07:27 2008
Categories: Homo sapiens | Protein complex | Bynum, J M. | Fletterick, R J. | Guy, R K. | Ingraham, H A. | Juzumiene, D. | Krylova, I N. | Lebedeva, L. | MacKay, J A. | Madauss, K. | Montana, V. | Moore, J. | Sablin, E P. | Suzawa, M. | Thornton, J W. | Waitt, G M. | Williams, J D. | Williams, S P. | Willson, T M. | Xu, R X. | P6L | Lrh-1 | Phosphatidylglycerol | Tif-1
