1z8s
From Proteopedia
(New page: 200px<br /><applet load="1z8s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z8s" /> '''DnaB binding domain of DnaG (P16) from Bacil...) |
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| - | [[Image:1z8s.gif|left|200px]]<br /><applet load="1z8s" size=" | + | [[Image:1z8s.gif|left|200px]]<br /><applet load="1z8s" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1z8s" /> | caption="1z8s" /> | ||
'''DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)'''<br /> | '''DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The helicase-primase interaction is a critical event in DNA replication | + | The helicase-primase interaction is a critical event in DNA replication and is mediated by a putative helicase-interaction domain within the primase. The solution structure of the helicase-interaction domain of DnaG reveals that it is made up of two independent subdomains: an N-terminal six-helix module and a C-terminal two-helix module that contains the residues of the primase previously identified as important in the interaction with the helicase. We show that the two-helix module alone is sufficient for strong binding between the primase and the helicase but fails to activate the helicase; both subdomains are required for helicase activation. The six-helix module of the primase has only one close structural homolog, the N-terminal domain of the corresponding helicase. This surprising structural relationship, coupled with the differences in surface properties of the two molecules, suggests how the helicase-interaction domain may perturb the structure of the helicase and lead to activation. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http:// | + | 1Z8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z8S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hounslow, A | + | [[Category: Hounslow, A M.]] |
[[Category: Soultanas, P.]] | [[Category: Soultanas, P.]] | ||
[[Category: Syson, K.]] | [[Category: Syson, K.]] | ||
[[Category: Thirlway, J.]] | [[Category: Thirlway, J.]] | ||
| - | [[Category: Waltho, J | + | [[Category: Waltho, J P.]] |
[[Category: two alpha helical sub-domains]] | [[Category: two alpha helical sub-domains]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:13:10 2008'' |
Revision as of 14:13, 21 February 2008
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DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)
Overview
The helicase-primase interaction is a critical event in DNA replication and is mediated by a putative helicase-interaction domain within the primase. The solution structure of the helicase-interaction domain of DnaG reveals that it is made up of two independent subdomains: an N-terminal six-helix module and a C-terminal two-helix module that contains the residues of the primase previously identified as important in the interaction with the helicase. We show that the two-helix module alone is sufficient for strong binding between the primase and the helicase but fails to activate the helicase; both subdomains are required for helicase activation. The six-helix module of the primase has only one close structural homolog, the N-terminal domain of the corresponding helicase. This surprising structural relationship, coupled with the differences in surface properties of the two molecules, suggests how the helicase-interaction domain may perturb the structure of the helicase and lead to activation.
About this Structure
1Z8S is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation., Syson K, Thirlway J, Hounslow AM, Soultanas P, Waltho JP, Structure. 2005 Apr;13(4):609-16. PMID:15837199
Page seeded by OCA on Thu Feb 21 16:13:10 2008
