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1z9e
From Proteopedia
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| - | [[Image:1z9e.gif|left|200px]]<br /> | + | [[Image:1z9e.gif|left|200px]]<br /><applet load="1z9e" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1z9e" size=" | + | |
caption="1z9e" /> | caption="1z9e" /> | ||
'''Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75'''<br /> | '''Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding | + | Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase (IN) in human cells. We have determined the NMR structure of the integrase-binding domain (IBD) in LEDGF and identified amino acid residues essential for the interaction. The IBD is a compact right-handed bundle composed of five alpha-helices. Based on folding topology, the IBD is structurally related to a diverse family of alpha-helical proteins that includes eukaryotic translation initiation factor eIF4G and karyopherin-beta. LEDGF residues essential for the interaction with IN were localized to interhelical loop regions of the bundle structure. Interaction-defective IN mutants were previously shown to cripple replication although they retained catalytic function. The initial structure determination of a host cell factor that tightly binds to a retroviral enzyme lays the groundwork for understanding enzyme-host interactions important for viral replication. |
==About this Structure== | ==About this Structure== | ||
| - | 1Z9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1Z9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9E OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Maertens, G.]] | [[Category: Maertens, G.]] | ||
[[Category: Rahman, S.]] | [[Category: Rahman, S.]] | ||
| - | [[Category: Sun, Z | + | [[Category: Sun, Z Y.J.]] |
[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
[[Category: heat repeat-like]] | [[Category: heat repeat-like]] | ||
[[Category: ledgf]] | [[Category: ledgf]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:13:25 2008'' |
Revision as of 14:13, 21 February 2008
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Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75
Overview
Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase (IN) in human cells. We have determined the NMR structure of the integrase-binding domain (IBD) in LEDGF and identified amino acid residues essential for the interaction. The IBD is a compact right-handed bundle composed of five alpha-helices. Based on folding topology, the IBD is structurally related to a diverse family of alpha-helical proteins that includes eukaryotic translation initiation factor eIF4G and karyopherin-beta. LEDGF residues essential for the interaction with IN were localized to interhelical loop regions of the bundle structure. Interaction-defective IN mutants were previously shown to cripple replication although they retained catalytic function. The initial structure determination of a host cell factor that tightly binds to a retroviral enzyme lays the groundwork for understanding enzyme-host interactions important for viral replication.
About this Structure
1Z9E is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75., Cherepanov P, Sun ZY, Rahman S, Maertens G, Wagner G, Engelman A, Nat Struct Mol Biol. 2005 Jun;12(6):526-32. Epub 2005 May 15. PMID:15895093
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