1zc8
From Proteopedia
(New page: 200px<br /><applet load="1zc8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zc8" /> '''Coordinates of tmRNA, SmpB, EF-Tu and h44 fi...) |
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| - | [[Image:1zc8.gif|left|200px]]<br /><applet load="1zc8" size=" | + | [[Image:1zc8.gif|left|200px]]<br /><applet load="1zc8" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex'''<br /> | '''Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are | + | Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are rescued by tmRNA, an approximately 300-nucleotide-long molecule that functions as both transfer RNA (tRNA) and mRNA. Translation then switches from the defective message to a short open reading frame on tmRNA that tags the defective nascent peptide chain for degradation. However, the mechanism by which tmRNA can enter and move through the ribosome is unknown. We present a cryo-electron microscopy study at approximately 13 to 15 angstroms of the entry of tmRNA into the ribosome. The structure reveals how tmRNA could move through the ribosome despite its complicated topology and also suggests roles for proteins S1 and SmpB in the function of tmRNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZC8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http:// | + | 1ZC8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZC8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: trans-translation]] | [[Category: trans-translation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:14:08 2008'' |
Revision as of 14:14, 21 February 2008
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Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex
Overview
Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are rescued by tmRNA, an approximately 300-nucleotide-long molecule that functions as both transfer RNA (tRNA) and mRNA. Translation then switches from the defective message to a short open reading frame on tmRNA that tags the defective nascent peptide chain for degradation. However, the mechanism by which tmRNA can enter and move through the ribosome is unknown. We present a cryo-electron microscopy study at approximately 13 to 15 angstroms of the entry of tmRNA into the ribosome. The structure reveals how tmRNA could move through the ribosome despite its complicated topology and also suggests roles for proteins S1 and SmpB in the function of tmRNA.
About this Structure
1ZC8 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Visualizing tmRNA entry into a stalled ribosome., Valle M, Gillet R, Kaur S, Henne A, Ramakrishnan V, Frank J, Science. 2003 Apr 4;300(5616):127-30. PMID:12677067
Page seeded by OCA on Thu Feb 21 16:14:08 2008
Categories: Protein complex | Thermus thermophilus | Frank, J. | Gillet, R. | Henne, A. | Kaur, S. | Ramakrishnan, V. | Valle, M. | 16s rrna | 30s | 70s | Cryo-em | Ef-tu | H44 | Smpb | Tmrna | Trans-translation
