2a0z
From Proteopedia
(New page: 200px<br /> <applet load="2a0z" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a0z, resolution 2.400Å" /> '''The molecular stru...) |
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| - | [[Image:2a0z.gif|left|200px]]<br /> | + | [[Image:2a0z.gif|left|200px]]<br /><applet load="2a0z" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2a0z" size=" | + | |
caption="2a0z, resolution 2.400Å" /> | caption="2a0z, resolution 2.400Å" /> | ||
'''The molecular structure of toll-like receptor 3 ligand binding domain'''<br /> | '''The molecular structure of toll-like receptor 3 ligand binding domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Innate immunity is the first line of defense against invading pathogens. | + | Innate immunity is the first line of defense against invading pathogens. Toll-like receptors (TLRs) act as sentinels of the innate immune system, sensing a variety of ligands from lipopolysaccharide to flagellin to dsRNA through their ligand-binding domain that is composed of leucine-rich repeats (LRRs). Ligand binding initiates a signaling cascade that leads to the up-regulation of inflammation mediators. In this study, we have expressed and crystallized the ectodomain (ECD) of human TLR3, which recognizes dsRNA, a molecular signature of viruses, and have determined the molecular structure to 2.4-A resolution. The overall horseshoe-shaped structure of the TLR3-ECD is formed by 23 repeating LRRs that are capped at each end by specialized non-LRR domains. The extensive beta-sheet on the molecule's concave surface forms a platform for several modifications, including insertions in the LRRs and 11 N-linked glycans. The TLR3-ECD structure indicates how LRR loops can establish distinct pathogen recognition receptors. |
==About this Structure== | ==About this Structure== | ||
| - | 2A0Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG, GLC, SO4 and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2A0Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A0Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Askins, J.]] | [[Category: Askins, J.]] | ||
| - | [[Category: Bell, J | + | [[Category: Bell, J K.]] |
[[Category: Botos, I.]] | [[Category: Botos, I.]] | ||
| - | [[Category: Davies, D | + | [[Category: Davies, D R.]] |
| - | [[Category: Hall, P | + | [[Category: Hall, P R.]] |
| - | [[Category: Segal, D | + | [[Category: Segal, D M.]] |
[[Category: Shiloach, J.]] | [[Category: Shiloach, J.]] | ||
[[Category: BME]] | [[Category: BME]] | ||
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[[Category: solenoid]] | [[Category: solenoid]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:32 2008'' |
Revision as of 14:22, 21 February 2008
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The molecular structure of toll-like receptor 3 ligand binding domain
Overview
Innate immunity is the first line of defense against invading pathogens. Toll-like receptors (TLRs) act as sentinels of the innate immune system, sensing a variety of ligands from lipopolysaccharide to flagellin to dsRNA through their ligand-binding domain that is composed of leucine-rich repeats (LRRs). Ligand binding initiates a signaling cascade that leads to the up-regulation of inflammation mediators. In this study, we have expressed and crystallized the ectodomain (ECD) of human TLR3, which recognizes dsRNA, a molecular signature of viruses, and have determined the molecular structure to 2.4-A resolution. The overall horseshoe-shaped structure of the TLR3-ECD is formed by 23 repeating LRRs that are capped at each end by specialized non-LRR domains. The extensive beta-sheet on the molecule's concave surface forms a platform for several modifications, including insertions in the LRRs and 11 N-linked glycans. The TLR3-ECD structure indicates how LRR loops can establish distinct pathogen recognition receptors.
About this Structure
2A0Z is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Full crystallographic information is available from OCA.
Reference
The molecular structure of the Toll-like receptor 3 ligand-binding domain., Bell JK, Botos I, Hall PR, Askins J, Shiloach J, Segal DM, Davies DR, Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10976-80. Epub 2005 Jul 25. PMID:16043704
Page seeded by OCA on Thu Feb 21 16:22:32 2008
Categories: Homo sapiens | Single protein | Askins, J. | Bell, J K. | Botos, I. | Davies, D R. | Hall, P R. | Segal, D M. | Shiloach, J. | BME | GLC | NAG | SO4 | Glycosylation | Leucine rich repeat | Solenoid
