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2a7o
From Proteopedia
(New page: 200px<br /> <applet load="2a7o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a7o" /> '''Solution Structure of the hSet2/HYPB SRI do...) |
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| - | [[Image:2a7o.gif|left|200px]]<br /> | + | [[Image:2a7o.gif|left|200px]]<br /><applet load="2a7o" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2a7o" size=" | + | |
caption="2a7o" /> | caption="2a7o" /> | ||
'''Solution Structure of the hSet2/HYPB SRI domain'''<br /> | '''Solution Structure of the hSet2/HYPB SRI domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The phosphorylation state of the C-terminal repeat domain (CTD) of the | + | The phosphorylation state of the C-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II changes as polymerase transcribes a gene, and the distinct forms of the phospho-CTD (PCTD) recruit different nuclear factors to elongating polymerase. The Set2 histone methyltransferase from yeast was recently shown to bind the PCTD of elongating RNA polymerase II by means of a novel domain termed the Set2-Rpb1 interacting (SRI) domain. Here, we report the solution structure of the SRI domain in human Set2 (hSRI domain), which adopts a left-turned three-helix bundle distinctly different from other structurally characterized PCTD-interacting domains. NMR titration experiments mapped the binding surface of the hSRI domain to helices 1 and 2, and Biacore binding studies showed that the domain binds preferably to [Ser-2 + Ser-5]-phosphorylated CTD peptides containing two or more heptad repeats. Point-mutagenesis studies identified five residues critical for PCTD binding. In view of the differential effects of these point mutations on binding to different CTD phosphopeptides, we propose a model for the hSRI domain interaction with the PCTD. |
==About this Structure== | ==About this Structure== | ||
| - | 2A7O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2A7O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Guan, Z.]] | [[Category: Guan, Z.]] | ||
[[Category: Li, M.]] | [[Category: Li, M.]] | ||
| - | [[Category: Phatnani, H | + | [[Category: Phatnani, H P.]] |
[[Category: Sage, H.]] | [[Category: Sage, H.]] | ||
[[Category: Zhou, P.]] | [[Category: Zhou, P.]] | ||
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[[Category: sri domain]] | [[Category: sri domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:24:31 2008'' |
Revision as of 14:24, 21 February 2008
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Solution Structure of the hSet2/HYPB SRI domain
Overview
The phosphorylation state of the C-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II changes as polymerase transcribes a gene, and the distinct forms of the phospho-CTD (PCTD) recruit different nuclear factors to elongating polymerase. The Set2 histone methyltransferase from yeast was recently shown to bind the PCTD of elongating RNA polymerase II by means of a novel domain termed the Set2-Rpb1 interacting (SRI) domain. Here, we report the solution structure of the SRI domain in human Set2 (hSRI domain), which adopts a left-turned three-helix bundle distinctly different from other structurally characterized PCTD-interacting domains. NMR titration experiments mapped the binding surface of the hSRI domain to helices 1 and 2, and Biacore binding studies showed that the domain binds preferably to [Ser-2 + Ser-5]-phosphorylated CTD peptides containing two or more heptad repeats. Point-mutagenesis studies identified five residues critical for PCTD binding. In view of the differential effects of these point mutations on binding to different CTD phosphopeptides, we propose a model for the hSRI domain interaction with the PCTD.
About this Structure
2A7O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1., Li M, Phatnani HP, Guan Z, Sage H, Greenleaf AL, Zhou P, Proc Natl Acad Sci U S A. 2005 Dec 6;102(49):17636-41. Epub 2005 Nov 28. PMID:16314571
Page seeded by OCA on Thu Feb 21 16:24:31 2008
Categories: Homo sapiens | Single protein | Greenleaf, A. | Guan, Z. | Li, M. | Phatnani, H P. | Sage, H. | Zhou, P. | Hset2 | Hsri | Pcap | Pcid | Phosphoctd associating protein | Set2 | Set2 rpb1-interacting domain | Sri | Sri domain
