2aqc
From Proteopedia
(New page: 200px<br /><applet load="2aqc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aqc" /> '''NMR Structural analysis of archaeal Nop10'''...) |
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| - | [[Image:2aqc.gif|left|200px]]<br /><applet load="2aqc" size=" | + | [[Image:2aqc.gif|left|200px]]<br /><applet load="2aqc" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''NMR Structural analysis of archaeal Nop10'''<br /> | '''NMR Structural analysis of archaeal Nop10'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Box H/ACA ribonucleoprotein particles (RNPs) catalyze RNA | + | Box H/ACA ribonucleoprotein particles (RNPs) catalyze RNA pseudouridylation and direct processing of ribosomal RNA, and are essential architectural components of vertebrate telomerases. H/ACA RNPs comprise four proteins and a multihelical RNA. Two proteins, Cbf5 and Nop10, suffice for basal enzymatic activity in an archaeal in vitro system. We now report their cocrystal structure at 1.95-A resolution. We find that archaeal Cbf5 can assemble with yeast Nop10 and with human telomerase RNA, consistent with the high sequence identity of the RNP components between archaea and eukarya. Thus, the Cbf5-Nop10 architecture is phylogenetically conserved. The structure shows how Nop10 buttresses the active site of Cbf5, and it reveals two basic troughs that bidirectionally extend the active site cleft. Mutagenesis results implicate an adjacent basic patch in RNA binding. This tripartite RNA-binding surface may function as a molecular bracket that organizes the multihelical H/ACA and telomerase RNAs. |
==About this Structure== | ==About this Structure== | ||
| - | 2AQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2AQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AQC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Amare, A | + | [[Category: Amare, A R.Ferre-D.]] |
[[Category: Hamma, T.]] | [[Category: Hamma, T.]] | ||
| - | [[Category: Reichow, S | + | [[Category: Reichow, S L.]] |
[[Category: Varani, G.]] | [[Category: Varani, G.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: zinc-ribbon]] | [[Category: zinc-ribbon]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:29:47 2008'' |
Revision as of 14:29, 21 February 2008
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NMR Structural analysis of archaeal Nop10
Overview
Box H/ACA ribonucleoprotein particles (RNPs) catalyze RNA pseudouridylation and direct processing of ribosomal RNA, and are essential architectural components of vertebrate telomerases. H/ACA RNPs comprise four proteins and a multihelical RNA. Two proteins, Cbf5 and Nop10, suffice for basal enzymatic activity in an archaeal in vitro system. We now report their cocrystal structure at 1.95-A resolution. We find that archaeal Cbf5 can assemble with yeast Nop10 and with human telomerase RNA, consistent with the high sequence identity of the RNP components between archaea and eukarya. Thus, the Cbf5-Nop10 architecture is phylogenetically conserved. The structure shows how Nop10 buttresses the active site of Cbf5, and it reveals two basic troughs that bidirectionally extend the active site cleft. Mutagenesis results implicate an adjacent basic patch in RNA binding. This tripartite RNA-binding surface may function as a molecular bracket that organizes the multihelical H/ACA and telomerase RNAs.
About this Structure
2AQC is a Single protein structure of sequence from Methanocaldococcus jannaschii with as ligand. Full crystallographic information is available from OCA.
Reference
The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs., Hamma T, Reichow SL, Varani G, Ferre-D'Amare AR, Nat Struct Mol Biol. 2005 Dec;12(12):1101-7. Epub 2005 Nov 15. PMID:16286935
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