2asb
From Proteopedia
(New page: 200px<br /><applet load="2asb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2asb, resolution 1.50Å" /> '''Structure of a Mycob...) |
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| - | [[Image:2asb.gif|left|200px]]<br /><applet load="2asb" size=" | + | [[Image:2asb.gif|left|200px]]<br /><applet load="2asb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2asb, resolution 1.50Å" /> | caption="2asb, resolution 1.50Å" /> | ||
'''Structure of a Mycobacterium tuberculosis NusA-RNA complex'''<br /> | '''Structure of a Mycobacterium tuberculosis NusA-RNA complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | NusA is a key regulator of bacterial transcriptional elongation, pausing, termination and antitermination, yet relatively little is known about the | + | NusA is a key regulator of bacterial transcriptional elongation, pausing, termination and antitermination, yet relatively little is known about the molecular basis of its activity in these fundamental processes. In Mycobacterium tuberculosis, NusA has been shown to bind with high affinity and specificity to BoxB-BoxA-BoxC antitermination sequences within the leader region of the single ribosomal RNA (rRNA) operon. We have determined high-resolution X-ray structures of a complex of NusA with two short oligo-ribonucleotides derived from the BoxC stem-loop motif and have characterised the interaction of NusA with a variety of RNAs derived from the antitermination region. These structures reveal the RNA bound in an extended conformation to a large interacting surface on both KH domains. Combining structural data with observed spectral and calorimetric changes, we now show that NusA binding destabilises secondary structure within rRNA antitermination sequences and propose a model where NusA functions as a chaperone for nascently forming RNA structures. |
==About this Structure== | ==About this Structure== | ||
| - | 2ASB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http:// | + | 2ASB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ASB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arnvig, K | + | [[Category: Arnvig, K B.]] |
[[Category: Beuth, B.]] | [[Category: Beuth, B.]] | ||
| - | [[Category: Martin, S | + | [[Category: Martin, S R.]] |
[[Category: Pennell, S.]] | [[Category: Pennell, S.]] | ||
| - | [[Category: Taylor, I | + | [[Category: Taylor, I A.]] |
[[Category: protein-rna complex]] | [[Category: protein-rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:30:32 2008'' |
Revision as of 14:30, 21 February 2008
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Structure of a Mycobacterium tuberculosis NusA-RNA complex
Overview
NusA is a key regulator of bacterial transcriptional elongation, pausing, termination and antitermination, yet relatively little is known about the molecular basis of its activity in these fundamental processes. In Mycobacterium tuberculosis, NusA has been shown to bind with high affinity and specificity to BoxB-BoxA-BoxC antitermination sequences within the leader region of the single ribosomal RNA (rRNA) operon. We have determined high-resolution X-ray structures of a complex of NusA with two short oligo-ribonucleotides derived from the BoxC stem-loop motif and have characterised the interaction of NusA with a variety of RNAs derived from the antitermination region. These structures reveal the RNA bound in an extended conformation to a large interacting surface on both KH domains. Combining structural data with observed spectral and calorimetric changes, we now show that NusA binding destabilises secondary structure within rRNA antitermination sequences and propose a model where NusA functions as a chaperone for nascently forming RNA structures.
About this Structure
2ASB is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
Structure of a Mycobacterium tuberculosis NusA-RNA complex., Beuth B, Pennell S, Arnvig KB, Martin SR, Taylor IA, EMBO J. 2005 Oct 19;24(20):3576-87. Epub 2005 Sep 29. PMID:16193062
Page seeded by OCA on Thu Feb 21 16:30:32 2008
