2bdz
From Proteopedia
(New page: 200px<br /><applet load="2bdz" size="350" color="white" frame="true" align="right" spinBox="true" caption="2bdz, resolution 2.100Å" /> '''Mexicain from Jacar...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical | + | Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress. |
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Jacaratia mexicana]] | [[Category: Jacaratia mexicana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Garcia-Ruiz, J | + | [[Category: Garcia-Ruiz, J M.]] |
| - | [[Category: Gavira, J | + | [[Category: Gavira, J A.]] |
| - | [[Category: Gonzalez-Ramirez, L | + | [[Category: Gonzalez-Ramirez, L A.]] |
| - | [[Category: Oliver-Salvador, M | + | [[Category: Oliver-Salvador, M C.]] |
[[Category: Soriano-Garcia, M.]] | [[Category: Soriano-Garcia, M.]] | ||
[[Category: E64]] | [[Category: E64]] | ||
| Line 24: | Line 24: | ||
[[Category: peptidase_c1]] | [[Category: peptidase_c1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:36:48 2008'' |
Revision as of 14:36, 21 February 2008
|
Mexicain from Jacaratia mexicana
Overview
Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress.
About this Structure
2BDZ is a Single protein structure of sequence from Jacaratia mexicana with as ligand. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:15502326
Page seeded by OCA on Thu Feb 21 16:36:48 2008
