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2bn2
From Proteopedia
(New page: 200px<br /><applet load="2bn2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bn2, resolution 2.80Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:2bn2.gif|left|200px]]<br /><applet load="2bn2" size=" | + | [[Image:2bn2.gif|left|200px]]<br /><applet load="2bn2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2bn2, resolution 2.80Å" /> | caption="2bn2, resolution 2.80Å" /> | ||
'''CRYSTAL STRUCTURE OF BOVINE NEUROPHYSIN II COMPLEXED WITH THE VASOPRESSIN ANALOGUE PHE-TYR AMIDE'''<br /> | '''CRYSTAL STRUCTURE OF BOVINE NEUROPHYSIN II COMPLEXED WITH THE VASOPRESSIN ANALOGUE PHE-TYR AMIDE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of a dipeptide complex of bovine neurophysin II has | + | The crystal structure of a dipeptide complex of bovine neurophysin II has been solved at 2.8 A resolution solely by using single-wavelength anomalous scattering data from a single iodinated derivative. The asymmetric unit is an elongated tetramer of dimensions 110 x 40 x 30 A, composed of two dimers related by pseudo twofold symmetry. Each monomer consists of two homologous layers, each with four antiparallel beta-strands. The two regions are connected by a helix followed by a long loop. Monomer-monomer contacts involve antiparallel beta-sheet interactions, which form a dimer with two layers of eight beta-strands. One peptide per monomer occupies the principal hormone-binding pocket formed by part of the amino-terminal region and parts of the connecting helix and loop, with binding to protein consistent with conclusions drawn from solution studies. Dimer-dimer contacts involve the Tyr49 region adjacent to this site. A fifth dipeptide, of unknown biological significance, helps to stabilize one of the monomer-monomer interfaces and the tetramer-tetramer network in the crystal. |
==About this Structure== | ==About this Structure== | ||
| - | 2BN2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure | + | 2BN2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry 1BN2. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Rose, J | + | [[Category: Rose, J P.]] |
| - | [[Category: Wang, B | + | [[Category: Wang, B C.]] |
[[Category: complex (protein/hormone)]] | [[Category: complex (protein/hormone)]] | ||
[[Category: hormone packaging]] | [[Category: hormone packaging]] | ||
[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:31 2008'' |
Revision as of 14:39, 21 February 2008
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CRYSTAL STRUCTURE OF BOVINE NEUROPHYSIN II COMPLEXED WITH THE VASOPRESSIN ANALOGUE PHE-TYR AMIDE
Overview
The crystal structure of a dipeptide complex of bovine neurophysin II has been solved at 2.8 A resolution solely by using single-wavelength anomalous scattering data from a single iodinated derivative. The asymmetric unit is an elongated tetramer of dimensions 110 x 40 x 30 A, composed of two dimers related by pseudo twofold symmetry. Each monomer consists of two homologous layers, each with four antiparallel beta-strands. The two regions are connected by a helix followed by a long loop. Monomer-monomer contacts involve antiparallel beta-sheet interactions, which form a dimer with two layers of eight beta-strands. One peptide per monomer occupies the principal hormone-binding pocket formed by part of the amino-terminal region and parts of the connecting helix and loop, with binding to protein consistent with conclusions drawn from solution studies. Dimer-dimer contacts involve the Tyr49 region adjacent to this site. A fifth dipeptide, of unknown biological significance, helps to stabilize one of the monomer-monomer interfaces and the tetramer-tetramer network in the crystal.
About this Structure
2BN2 is a Single protein structure of sequence from Bos taurus. This structure supersedes the now removed PDB entry 1BN2. Full crystallographic information is available from OCA.
Reference
Crystal structure of a bovine neurophysin II dipeptide complex at 2.8 A determined from the single-wavelength anomalous scattering signal of an incorporated iodine atom., Chen LQ, Rose JP, Breslow E, Yang D, Chang WR, Furey WF Jr, Sax M, Wang BC, Proc Natl Acad Sci U S A. 1991 May 15;88(10):4240-4. PMID:2034668
Page seeded by OCA on Thu Feb 21 16:39:31 2008
