2boe
From Proteopedia
(New page: 200px<br /><applet load="2boe" size="450" color="white" frame="true" align="right" spinBox="true" caption="2boe, resolution 1.15Å" /> '''CATALYTIC DOMAIN OF ...) |
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| - | [[Image:2boe.gif|left|200px]]<br /><applet load="2boe" size=" | + | [[Image:2boe.gif|left|200px]]<br /><applet load="2boe" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2boe, resolution 1.15Å" /> | caption="2boe, resolution 1.15Å" /> | ||
'''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA'''<br /> | '''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 | + | Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution. |
==About this Structure== | ==About this Structure== | ||
| - | 2BOE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http:// | + | 2BOE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BOE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Driguez, H.]] | [[Category: Driguez, H.]] | ||
[[Category: Dultz, E.]] | [[Category: Dultz, E.]] | ||
| - | [[Category: Irwin, D | + | [[Category: Irwin, D C.]] |
| - | [[Category: Jones, T | + | [[Category: Jones, T A.]] |
| - | [[Category: Larsson, A | + | [[Category: Larsson, A M.]] |
[[Category: Roos, A.]] | [[Category: Roos, A.]] | ||
| - | [[Category: Wilson, D | + | [[Category: Wilson, D B.]] |
[[Category: carbohydrate metabolism]] | [[Category: carbohydrate metabolism]] | ||
[[Category: cellulose degradation]] | [[Category: cellulose degradation]] | ||
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[[Category: tim a/b fold]] | [[Category: tim a/b fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:56 2008'' |
Revision as of 14:39, 21 February 2008
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CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA
Overview
Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution.
About this Structure
2BOE is a Single protein structure of sequence from Thermobifida fusca. Active as Cellulase, with EC number 3.2.1.4 Full crystallographic information is available from OCA.
Reference
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:16185060
Page seeded by OCA on Thu Feb 21 16:39:56 2008
Categories: Cellulase | Single protein | Thermobifida fusca | Bergfors, T. | Driguez, H. | Dultz, E. | Irwin, D C. | Jones, T A. | Larsson, A M. | Roos, A. | Wilson, D B. | Carbohydrate metabolism | Cellulose degradation | Endoglucanase | Glycosidase | Glycoside hydrolase family 6 | Hydrolase | Methyl cellobiosyl-4-thio-beta-cellobioside | Polysaccharide degradation | Tim a/b fold
