2cii
From Proteopedia
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==Overview== | ==Overview== | ||
| - | In the absence of bound peptide ligands, major histocompatibility complex | + | In the absence of bound peptide ligands, major histocompatibility complex (MHC) class I molecules are unstable. In an attempt to determine the minimum requirement for peptide-dependent MHC class I stabilization, we have used short synthetic peptides derived from the Sendai virus nucleoprotein epitope (residues 324-332, 1FAPGNYPAL9) to promote its folding in vitro of H-2D(b). We found that H-2D(b) can be stabilized by the pentapeptide 5NYPAL9, which is equivalent to the C-terminal portion of the optimal nonapeptide and includes both the P5 and P9 anchor residues. We have crystallized the complex of the H-2D(b) molecule with the pentamer and determined the structure to show how a quasi-stable MHC class I molecule can be formed by occupancy of a single binding pocket in the peptide-binding groove. |
==Disease== | ==Disease== | ||
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[[Category: Elliott, T.]] | [[Category: Elliott, T.]] | ||
[[Category: Glithero, A.]] | [[Category: Glithero, A.]] | ||
| - | [[Category: Jones, E | + | [[Category: Jones, E Y.]] |
[[Category: Kojima, M.]] | [[Category: Kojima, M.]] | ||
[[Category: Tormo, J.]] | [[Category: Tormo, J.]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:02 2008'' |
Revision as of 14:49, 21 February 2008
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THE CRYSTAL STRUCTURE OF H-2DB COMPLEXED WITH A PARTIAL PEPTIDE EPITOPE SUGGESTS AN MHC CLASS I ASSEMBLY-INTERMEDIATE
Contents |
Overview
In the absence of bound peptide ligands, major histocompatibility complex (MHC) class I molecules are unstable. In an attempt to determine the minimum requirement for peptide-dependent MHC class I stabilization, we have used short synthetic peptides derived from the Sendai virus nucleoprotein epitope (residues 324-332, 1FAPGNYPAL9) to promote its folding in vitro of H-2D(b). We found that H-2D(b) can be stabilized by the pentapeptide 5NYPAL9, which is equivalent to the C-terminal portion of the optimal nonapeptide and includes both the P5 and P9 anchor residues. We have crystallized the complex of the H-2D(b) molecule with the pentamer and determined the structure to show how a quasi-stable MHC class I molecule can be formed by occupancy of a single binding pocket in the peptide-binding groove.
Disease
Known disease associated with this structure: Hypoproteinemia, hypercatabolic OMIM:[109700]
About this Structure
2CII is a Protein complex structure of sequences from Homo sapiens and Mus musculus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of H-2D(b) complexed with a partial peptide epitope suggests a major histocompatibility complex class I assembly intermediate., Glithero A, Tormo J, Doering K, Kojima M, Jones EY, Elliott T, J Biol Chem. 2006 May 5;281(18):12699-704. Epub 2006 Feb 14. PMID:16478731
Page seeded by OCA on Thu Feb 21 16:49:02 2008
Categories: Homo sapiens | Mus musculus | Protein complex | Doering, K. | Elliott, T. | Glithero, A. | Jones, E Y. | Kojima, M. | Tormo, J. | GOL | Complex (antigen/peptide) | Glycoprotein | Immune response | Immunoglobulin domain | Membrane | Mhc class i | Mhc i | Peptide binding | Pyrrolidone carboxylic acid | Sendai virus | Transmembrane
