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3kvc
From Proteopedia
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{{STRUCTURE_3kvc| PDB=3kvc | SCENE= }} | {{STRUCTURE_3kvc| PDB=3kvc | SCENE= }} | ||
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===Crystal structure of bovine RPE65 at 1.9 angstrom resolution=== | ===Crystal structure of bovine RPE65 at 1.9 angstrom resolution=== | ||
| + | {{ABSTRACT_PUBMED_20100834}} | ||
| - | + | ==Function== | |
| - | + | [[http://www.uniprot.org/uniprot/RPE65_BOVIN RPE65_BOVIN]] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.<ref>PMID:16096063</ref> <ref>PMID:19805034</ref> <ref>PMID:20100834</ref> | |
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==About this Structure== | ==About this Structure== | ||
| - | + | [[3kvc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVC OCA]. | |
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID: | + | <ref group="xtra">PMID:020100834</ref><references group="xtra"/><references/> |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Golczak, M.]] | [[Category: Golczak, M.]] | ||
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[[Category: Palczewski, K.]] | [[Category: Palczewski, K.]] | ||
[[Category: 7-bladed beta-propeller]] | [[Category: 7-bladed beta-propeller]] | ||
| - | [[Category: Acetylation]] | ||
[[Category: Cell membrane]] | [[Category: Cell membrane]] | ||
| - | [[Category: Cytoplasm]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Iron]] | [[Category: Iron]] | ||
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[[Category: Sensory transduction]] | [[Category: Sensory transduction]] | ||
[[Category: Vision]] | [[Category: Vision]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 12 10:36:00 2010'' | ||
Revision as of 20:31, 4 April 2013
Contents |
Crystal structure of bovine RPE65 at 1.9 angstrom resolution
Template:ABSTRACT PUBMED 20100834
Function
[RPE65_BOVIN] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.[1] [2] [3]
About this Structure
3kvc is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
- Golczak M, Kiser PD, Lodowski DT, Maeda A, Palczewski K. Importance of membrane structural integrity for RPE65 retinoid isomerization activity. J Biol Chem. 2010 Mar 26;285(13):9667-82. Epub 2010 Jan 25. PMID:20100834 doi:10.1074/jbc.M109.063941
- ↑ Jin M, Li S, Moghrabi WN, Sun H, Travis GH. Rpe65 is the retinoid isomerase in bovine retinal pigment epithelium. Cell. 2005 Aug 12;122(3):449-59. PMID:16096063 doi:10.1016/j.cell.2005.06.042
- ↑ Kiser PD, Golczak M, Lodowski DT, Chance MR, Palczewski K. Crystal structure of native RPE65, the retinoid isomerase of the visual cycle. Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17325-30. Epub 2009 Oct 5. PMID:19805034
- ↑ Golczak M, Kiser PD, Lodowski DT, Maeda A, Palczewski K. Importance of membrane structural integrity for RPE65 retinoid isomerization activity. J Biol Chem. 2010 Mar 26;285(13):9667-82. Epub 2010 Jan 25. PMID:20100834 doi:10.1074/jbc.M109.063941
Categories: Bos taurus | Golczak, M. | Kiser, P D. | Lodowski, D T. | Palczewski, K. | 7-bladed beta-propeller | Cell membrane | Hydrolase | Iron | Isomerase | Lipoprotein | Membrane | Metal-binding | Monotopic membrane protein | Non-heme iron protein | Palmitate | Phosphoprotein | Sensory transduction | Vision
