2f5o
From Proteopedia
(New page: 200px<br /><applet load="2f5o" size="350" color="white" frame="true" align="right" spinBox="true" caption="2f5o, resolution 2.05Å" /> '''MutM crosslinked to ...) |
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==Overview== | ==Overview== | ||
| - | DNA glycosylases must interrogate millions of base pairs of undamaged DNA | + | DNA glycosylases must interrogate millions of base pairs of undamaged DNA in order to locate and then excise one damaged nucleobase. The nature of this search process remains poorly understood. Here we report the use of disulfide cross-linking (DXL) technology to obtain structures of a bacterial DNA glycosylase, MutM, interrogating undamaged DNA. These structures, solved to 2.0 angstrom resolution, reveal the nature of the search process: The protein inserts a probe residue into the helical stack and severely buckles the target base pair, which remains intrahelical. MutM therefore actively interrogates the intact DNA helix while searching for damage. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Banerjee, A.]] | [[Category: Banerjee, A.]] | ||
| - | [[Category: Santos, W | + | [[Category: Santos, W L.]] |
| - | [[Category: Verdine, G | + | [[Category: Verdine, G L.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: dna repair]] | [[Category: dna repair]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:02 2008'' |
Revision as of 15:18, 21 February 2008
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MutM crosslinked to undamaged DNA sampling G:C base pair IC3
Overview
DNA glycosylases must interrogate millions of base pairs of undamaged DNA in order to locate and then excise one damaged nucleobase. The nature of this search process remains poorly understood. Here we report the use of disulfide cross-linking (DXL) technology to obtain structures of a bacterial DNA glycosylase, MutM, interrogating undamaged DNA. These structures, solved to 2.0 angstrom resolution, reveal the nature of the search process: The protein inserts a probe residue into the helical stack and severely buckles the target base pair, which remains intrahelical. MutM therefore actively interrogates the intact DNA helix while searching for damage.
About this Structure
2F5O is a Single protein structure of sequence from Geobacillus stearothermophilus with and as ligands. Active as DNA-formamidopyrimidine glycosylase, with EC number 3.2.2.23 Full crystallographic information is available from OCA.
Reference
Structure of a DNA glycosylase searching for lesions., Banerjee A, Santos WL, Verdine GL, Science. 2006 Feb 24;311(5764):1153-7. PMID:16497933
Page seeded by OCA on Thu Feb 21 17:18:02 2008
