2f8x
From Proteopedia
(New page: 200px<br /> <applet load="2f8x" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f8x, resolution 3.250Å" /> '''Crystal structure ...) |
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| - | [[Image:2f8x.gif|left|200px]]<br /> | + | [[Image:2f8x.gif|left|200px]]<br /><applet load="2f8x" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2f8x, resolution 3.250Å" /> | caption="2f8x, resolution 3.250Å" /> | ||
'''Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence'''<br /> | '''Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Notch receptors transduce essential developmental signals between | + | Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2F8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2F8X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Blacklow, S | + | [[Category: Blacklow, S C.]] |
[[Category: Nam, Y.]] | [[Category: Nam, Y.]] | ||
[[Category: Sliz, P.]] | [[Category: Sliz, P.]] | ||
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[[Category: rel-homology region]] | [[Category: rel-homology region]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:55 2008'' |
Revision as of 15:18, 21 February 2008
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Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence
Contents |
Overview
Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes.
Disease
Known diseases associated with this structure: Aortic valve disease OMIM:[190198], Leukemia, T-cell acute lymphoblastic OMIM:[190198]
About this Structure
2F8X is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes., Nam Y, Sliz P, Song L, Aster JC, Blacklow SC, Cell. 2006 Mar 10;124(5):973-83. PMID:16530044
Page seeded by OCA on Thu Feb 21 17:18:55 2008
Categories: Homo sapiens | Protein complex | Blacklow, S C. | Nam, Y. | Sliz, P. | Ankyrin repeats | Csl | Hes-1 | Mastermind | Notch | Rel-homology region
