This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2fk6
From Proteopedia
(New page: 200px<br /><applet load="2fk6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fk6, resolution 2.90Å" /> '''Crystal Structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:2fk6.gif|left|200px]]<br /><applet load="2fk6" size=" | + | [[Image:2fk6.gif|left|200px]]<br /><applet load="2fk6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2fk6, resolution 2.90Å" /> | caption="2fk6, resolution 2.90Å" /> | ||
'''Crystal Structure of RNAse Z/tRNA(Thr) complex'''<br /> | '''Crystal Structure of RNAse Z/tRNA(Thr) complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The highly conserved ribonuclease RNase Z catalyzes the endonucleolytic | + | The highly conserved ribonuclease RNase Z catalyzes the endonucleolytic removal of the 3' extension of the majority of tRNA precursors. Here we present the structure of the complex between Bacillus subtilis RNase Z and tRNA(Thr), the first structure of a ribonucleolytic processing enzyme bound to tRNA. Binding of tRNA to RNase Z causes conformational changes in both partners to promote reorganization of the catalytic site and tRNA cleavage. |
==About this Structure== | ==About this Structure== | ||
| - | 2FK6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with ZN, MES and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_Z Ribonuclease Z], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.11 3.1.26.11] Full crystallographic information is available from [http:// | + | 2FK6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MES:'>MES</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_Z Ribonuclease Z], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.11 3.1.26.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FK6 OCA]. |
==Reference== | ==Reference== | ||
| Line 17: | Line 17: | ||
[[Category: Mathy, N.]] | [[Category: Mathy, N.]] | ||
[[Category: Pellegrini, O.]] | [[Category: Pellegrini, O.]] | ||
| - | [[Category: Sierra-Gallay, I | + | [[Category: Sierra-Gallay, I Li de la.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: MES]] | [[Category: MES]] | ||
| Line 23: | Line 23: | ||
[[Category: protein-trna complex; zinc-dependent metal hydrolase; trna maturase]] | [[Category: protein-trna complex; zinc-dependent metal hydrolase; trna maturase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:22:11 2008'' |
Revision as of 15:22, 21 February 2008
|
Crystal Structure of RNAse Z/tRNA(Thr) complex
Overview
The highly conserved ribonuclease RNase Z catalyzes the endonucleolytic removal of the 3' extension of the majority of tRNA precursors. Here we present the structure of the complex between Bacillus subtilis RNase Z and tRNA(Thr), the first structure of a ribonucleolytic processing enzyme bound to tRNA. Binding of tRNA to RNase Z causes conformational changes in both partners to promote reorganization of the catalytic site and tRNA cleavage.
About this Structure
2FK6 is a Protein complex structure of sequences from Bacillus subtilis with , and as ligands. Active as Ribonuclease Z, with EC number 3.1.26.11 Full crystallographic information is available from OCA.
Reference
Structure of the ubiquitous 3' processing enzyme RNase Z bound to transfer RNA., Li de la Sierra-Gallay I, Mathy N, Pellegrini O, Condon C, Nat Struct Mol Biol. 2006 Apr;13(4):376-7. Epub 2006 Mar 5. PMID:16518398
Page seeded by OCA on Thu Feb 21 17:22:11 2008
