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2id8

From Proteopedia

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(New page: 200px<br /><applet load="2id8" size="450" color="white" frame="true" align="right" spinBox="true" caption="2id8, resolution 1.27&Aring;" /> '''Crystal structure of...)
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[[Image:2id8.gif|left|200px]]<br /><applet load="2id8" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2id8, resolution 1.27&Aring;" />
caption="2id8, resolution 1.27&Aring;" />
'''Crystal structure of Proteinase K'''<br />
'''Crystal structure of Proteinase K'''<br />
==Overview==
==Overview==
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X-ray single-wavelength anomalous diffraction (SAD) data from a crystal of, proteinase K were collected using synchrotron radiation of 0.98 A, wavelength at SER-CAT 22-ID beamline, Advanced Photon Source, Argonne, National Laboratory. At this wavelength, the expected Bijvoet ratio, resulting from the presence of one calcium, one chloride and ten S atoms, in the 279-residue protein is extremely small at approximately 0.46%. The, direct-methods program SHELXD located 11 anomalous sites using data, truncated to 2 A resolution. SHELXE was used to produce an easily, interpretable electron-density map. This study shows that an accurate, beamline and a good-quality crystal provide the possibility of, successfully using a very weak anomalous signal of sulfur measured at a, short wavelength for phasing a protein structure, even if a small degree, of radiation damage is present.
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X-ray single-wavelength anomalous diffraction (SAD) data from a crystal of proteinase K were collected using synchrotron radiation of 0.98 A wavelength at SER-CAT 22-ID beamline, Advanced Photon Source, Argonne National Laboratory. At this wavelength, the expected Bijvoet ratio resulting from the presence of one calcium, one chloride and ten S atoms in the 279-residue protein is extremely small at approximately 0.46%. The direct-methods program SHELXD located 11 anomalous sites using data truncated to 2 A resolution. SHELXE was used to produce an easily interpretable electron-density map. This study shows that an accurate beamline and a good-quality crystal provide the possibility of successfully using a very weak anomalous signal of sulfur measured at a short wavelength for phasing a protein structure, even if a small degree of radiation damage is present.
==About this Structure==
==About this Structure==
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2ID8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album] with CA, NO3, CL and 2DB as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ID8 OCA].
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2ID8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=NO3:'>NO3</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=2DB:'>2DB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ID8 OCA].
==Reference==
==Reference==
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[[Category: proteinase]]
[[Category: proteinase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:15:22 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:51:27 2008''

Revision as of 15:51, 21 February 2008

Image:2id8.gif

2id8, resolution 1.27Å

Drag the structure with the mouse to rotate

Crystal structure of Proteinase K

Overview

X-ray single-wavelength anomalous diffraction (SAD) data from a crystal of proteinase K were collected using synchrotron radiation of 0.98 A wavelength at SER-CAT 22-ID beamline, Advanced Photon Source, Argonne National Laboratory. At this wavelength, the expected Bijvoet ratio resulting from the presence of one calcium, one chloride and ten S atoms in the 279-residue protein is extremely small at approximately 0.46%. The direct-methods program SHELXD located 11 anomalous sites using data truncated to 2 A resolution. SHELXE was used to produce an easily interpretable electron-density map. This study shows that an accurate beamline and a good-quality crystal provide the possibility of successfully using a very weak anomalous signal of sulfur measured at a short wavelength for phasing a protein structure, even if a small degree of radiation damage is present.

About this Structure

2ID8 is a Single protein structure of sequence from Engyodontium album with , , and as ligands. Active as Peptidase K, with EC number 3.4.21.64 Full crystallographic information is available from OCA.

Reference

What can be done with a good crystal and an accurate beamline?, Wang J, Dauter M, Dauter Z, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1475-83. Epub 2006, Nov 23. PMID:17139083

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