2il9
From Proteopedia
(New page: 200px<br /><applet load="2il9" size="350" color="white" frame="true" align="right" spinBox="true" caption="2il9, resolution 3.100Å" /> '''Crystal Structure o...) |
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==Overview== | ==Overview== | ||
| - | Canonical cap-dependent translation initiation requires a large number of | + | Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo-electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA. |
==About this Structure== | ==About this Structure== | ||
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Structural basis for ribosome recruitment and manipulation by a viral IRES RNA., Pfingsten JS, Costantino DA, Kieft JS, Science. 2006 Dec 1;314(5804):1450-4. Epub 2006 Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17124290 17124290] | Structural basis for ribosome recruitment and manipulation by a viral IRES RNA., Pfingsten JS, Costantino DA, Kieft JS, Science. 2006 Dec 1;314(5804):1450-4. Epub 2006 Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17124290 17124290] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Costantino, D | + | [[Category: Costantino, D A.]] |
| - | [[Category: Kieft, J | + | [[Category: Kieft, J S.]] |
| - | [[Category: Pfingsten, J | + | [[Category: Pfingsten, J S.]] |
[[Category: rna; ires; ribosomal binding domain; psiv; under wound helix; double-nested pseudoknot]] | [[Category: rna; ires; ribosomal binding domain; psiv; under wound helix; double-nested pseudoknot]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:53:44 2008'' |
Revision as of 15:53, 21 February 2008
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Crystal Structure of Plautia Stali Intestine Virus Intergenic Region Internal Ribosome Entry Site Ribosomal Binding Domain RNA at 3.1 Angstroms
Overview
Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo-electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.
About this Structure
2IL9 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural basis for ribosome recruitment and manipulation by a viral IRES RNA., Pfingsten JS, Costantino DA, Kieft JS, Science. 2006 Dec 1;314(5804):1450-4. Epub 2006 Nov 23. PMID:17124290
Page seeded by OCA on Thu Feb 21 17:53:44 2008
