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2jex
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Redox changes are one of the factors that influence cell-cycle progression | + | Redox changes are one of the factors that influence cell-cycle progression and that control the processes of cellular proliferation, differentiation, senescence and apoptosis. Proteins regulated through redox-sensitive cysteines have been characterized but specific 'sulphydryl switches' in replication proteins remain to be identified. In bovine papillomavirus type-1, DNA replication begins when the viral transcription factor E2 recruits the viral initiator protein E1 to the origin of DNA replication (ori). Here we show that a novel dimerization interface in the E2 transcription activation domain is stabilized by a disulphide bond. Oxidative cross-linking via Cys57 sequesters the interaction surface between E1 and E2, preventing pre-initiation and replication initiation complex formation. Our data demonstrate that as well as a mechanism for regulating DNA binding, redox reactions can control replication by modulating the tertiary structure of critical protein factors using a specific redox sensor. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Bovine papillomavirus type 1]] | [[Category: Bovine papillomavirus type 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Antson, A | + | [[Category: Antson, A A.]] |
[[Category: Ortiz-Lombardia, M.]] | [[Category: Ortiz-Lombardia, M.]] | ||
| - | [[Category: Sanders, C | + | [[Category: Sanders, C M.]] |
| - | [[Category: Seavers, P | + | [[Category: Seavers, P R.]] |
[[Category: Sizov, D.]] | [[Category: Sizov, D.]] | ||
[[Category: activator]] | [[Category: activator]] | ||
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[[Category: viral transcription factor]] | [[Category: viral transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:02:31 2008'' |
Revision as of 16:02, 21 February 2008
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TRANSCRIPTION ACTIVATOR STRUCTURE REVEALS REDOX CONTROL OF A REPLICATION INITIATION REACTION
Overview
Redox changes are one of the factors that influence cell-cycle progression and that control the processes of cellular proliferation, differentiation, senescence and apoptosis. Proteins regulated through redox-sensitive cysteines have been characterized but specific 'sulphydryl switches' in replication proteins remain to be identified. In bovine papillomavirus type-1, DNA replication begins when the viral transcription factor E2 recruits the viral initiator protein E1 to the origin of DNA replication (ori). Here we show that a novel dimerization interface in the E2 transcription activation domain is stabilized by a disulphide bond. Oxidative cross-linking via Cys57 sequesters the interaction surface between E1 and E2, preventing pre-initiation and replication initiation complex formation. Our data demonstrate that as well as a mechanism for regulating DNA binding, redox reactions can control replication by modulating the tertiary structure of critical protein factors using a specific redox sensor.
About this Structure
2JEX is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.
Reference
Transcription activator structure reveals redox control of a replication initiation reaction., Sanders CM, Sizov D, Seavers PR, Ortiz-Lombardia M, Antson AA, Nucleic Acids Res. 2007;35(10):3504-15. Epub 2007 May 3. PMID:17478495
Page seeded by OCA on Thu Feb 21 18:02:31 2008
Categories: Bovine papillomavirus type 1 | Single protein | Antson, A A. | Ortiz-Lombardia, M. | Sanders, C M. | Seavers, P R. | Sizov, D. | Activator | Bovine papillomavirus | Dna replication | Dna-binding | E1 | E2 | Early protein | Nuclear protein | Oxidation | Phosphorylation | Redox control | Replication initiation | Repressor | Transcription | Transcription regulation | Viral transcription factor
