2jgo
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Arsenic, a contaminant of water supplies worldwide, is one of the most | + | Arsenic, a contaminant of water supplies worldwide, is one of the most toxic inorganic ions. Despite arsenic's health impact, there is relatively little structural detail known about its interactions with proteins. Bacteria such as Escherichia coli have evolved arsenic resistance using the Ars operon that is regulated by ArsR, a repressor protein that dissociates from DNA when As(III) binds. This protein undergoes a critical conformational change upon binding As(III) with three cysteine residues. Unfortunately, structures of ArsR with or without As(III) have not been reported. Alternatively, de novo designed peptides can bind As(III) in an endo configuration within a thiolate-rich environment consistent with that proposed for both ArsR and ArsD. We report the structure of the As(III) complex of Coil Ser L9C to a 1.8-A resolution, providing x-ray characterization of As(III) in a Tris thiolate protein environment and allowing a structural basis by which to understand arsenated ArsR. |
==About this Structure== | ==About this Structure== | ||
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Identifying important structural characteristics of arsenic resistance proteins by using designed three-stranded coiled coils., Touw DS, Nordman CE, Stuckey JA, Pecoraro VL, Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):11969-74. Epub 2007 Jul 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17609383 17609383] | Identifying important structural characteristics of arsenic resistance proteins by using designed three-stranded coiled coils., Touw DS, Nordman CE, Stuckey JA, Pecoraro VL, Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):11969-74. Epub 2007 Jul 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17609383 17609383] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Nordman, C | + | [[Category: Nordman, C E.]] |
| - | [[Category: Pecoraro, V | + | [[Category: Pecoraro, V L.]] |
| - | [[Category: Stuckey, J | + | [[Category: Stuckey, J A.]] |
| - | [[Category: Touw, D | + | [[Category: Touw, D S.]] |
[[Category: ACE]] | [[Category: ACE]] | ||
[[Category: ARS]] | [[Category: ARS]] | ||
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[[Category: three-stranded coiled coil]] | [[Category: three-stranded coiled coil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:03:08 2008'' |
Revision as of 16:03, 21 February 2008
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STUCTURE OF THE ARSENATED DE NOVO DESIGNED PEPTIDE COIL SER L9C
Overview
Arsenic, a contaminant of water supplies worldwide, is one of the most toxic inorganic ions. Despite arsenic's health impact, there is relatively little structural detail known about its interactions with proteins. Bacteria such as Escherichia coli have evolved arsenic resistance using the Ars operon that is regulated by ArsR, a repressor protein that dissociates from DNA when As(III) binds. This protein undergoes a critical conformational change upon binding As(III) with three cysteine residues. Unfortunately, structures of ArsR with or without As(III) have not been reported. Alternatively, de novo designed peptides can bind As(III) in an endo configuration within a thiolate-rich environment consistent with that proposed for both ArsR and ArsD. We report the structure of the As(III) complex of Coil Ser L9C to a 1.8-A resolution, providing x-ray characterization of As(III) in a Tris thiolate protein environment and allowing a structural basis by which to understand arsenated ArsR.
About this Structure
2JGO is a Single protein structure of sequence from [1] with , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Identifying important structural characteristics of arsenic resistance proteins by using designed three-stranded coiled coils., Touw DS, Nordman CE, Stuckey JA, Pecoraro VL, Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):11969-74. Epub 2007 Jul 3. PMID:17609383
Page seeded by OCA on Thu Feb 21 18:03:08 2008
