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2jpp
From Proteopedia
(New page: 200px<br /><applet load="2jpp" size="350" color="white" frame="true" align="right" spinBox="true" caption="2jpp" /> '''Structural basis of RsmA/CsrA RNA recognitio...) |
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==Overview== | ==Overview== | ||
| - | Proteins of the RsmA/CsrA family are global translational regulators in | + | Proteins of the RsmA/CsrA family are global translational regulators in many bacterial species. We have determined the solution structure of a complex formed between the RsmE protein, a member of this family from Pseudomonas fluorescens, and a target RNA encompassing the ribosome-binding site of the hcnA gene. The RsmE homodimer with its two RNA-binding sites makes optimal contact with an 5'-A/UCANGGANGU/A-3' sequence in the mRNA. When tightly gripped by RsmE, the ANGGAN core folds into a loop, favoring the formation of a 3-base-pair stem by flanking nucleotides. We validated these findings by in vivo and in vitro mutational analyses. The structure of the complex explains well how, by sequestering the Shine-Dalgarno sequence, the RsmA/CsrA proteins repress translation. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Pseudomonas fluorescens]] | [[Category: Pseudomonas fluorescens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Allain, F | + | [[Category: Allain, F H.T.]] |
[[Category: Duss, O.]] | [[Category: Duss, O.]] | ||
[[Category: Haas, D.]] | [[Category: Haas, D.]] | ||
[[Category: Jelesarov, I.]] | [[Category: Jelesarov, I.]] | ||
[[Category: Lapouge, K.]] | [[Category: Lapouge, K.]] | ||
| - | [[Category: Oberstrass, F | + | [[Category: Oberstrass, F C.]] |
[[Category: Schubert, M.]] | [[Category: Schubert, M.]] | ||
[[Category: csra]] | [[Category: csra]] | ||
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[[Category: translation/rna complex]] | [[Category: translation/rna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:04:58 2008'' |
Revision as of 16:05, 21 February 2008
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Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA
Overview
Proteins of the RsmA/CsrA family are global translational regulators in many bacterial species. We have determined the solution structure of a complex formed between the RsmE protein, a member of this family from Pseudomonas fluorescens, and a target RNA encompassing the ribosome-binding site of the hcnA gene. The RsmE homodimer with its two RNA-binding sites makes optimal contact with an 5'-A/UCANGGANGU/A-3' sequence in the mRNA. When tightly gripped by RsmE, the ANGGAN core folds into a loop, favoring the formation of a 3-base-pair stem by flanking nucleotides. We validated these findings by in vivo and in vitro mutational analyses. The structure of the complex explains well how, by sequestering the Shine-Dalgarno sequence, the RsmA/CsrA proteins repress translation.
About this Structure
2JPP is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.
Reference
Molecular basis of messenger RNA recognition by the specific bacterial repressing clamp RsmA/CsrA., Schubert M, Lapouge K, Duss O, Oberstrass FC, Jelesarov I, Haas D, Allain FH, Nat Struct Mol Biol. 2007 Sep;14(9):807-13. Epub 2007 Aug 19. PMID:17704818
Page seeded by OCA on Thu Feb 21 18:04:58 2008
