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2mjp
From Proteopedia
(New page: 200px<br /><applet load="2mjp" size="450" color="white" frame="true" align="right" spinBox="true" caption="2mjp, resolution 2.2Å" /> '''STRUCTURE-BASED IDENT...) |
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| - | [[Image:2mjp.gif|left|200px]]<br /><applet load="2mjp" size=" | + | [[Image:2mjp.gif|left|200px]]<br /><applet load="2mjp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2mjp, resolution 2.2Å" /> | caption="2mjp, resolution 2.2Å" /> | ||
'''STRUCTURE-BASED IDENTIFICATION OF THE BIOCHEMICAL FUNCTION OF A HYPOTHETICAL PROTEIN FROM METHANOCOCCUS JANNASCHII:MJ0226'''<br /> | '''STRUCTURE-BASED IDENTIFICATION OF THE BIOCHEMICAL FUNCTION OF A HYPOTHETICAL PROTEIN FROM METHANOCOCCUS JANNASCHII:MJ0226'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Almost half of the entire set of predicted genomic products from | + | Almost half of the entire set of predicted genomic products from Methanococcus jannaschii are classified as functionally unknown hypothetical proteins. We present a structure-based identification of the biochemical function of a protein with an as yet unknown function from a M. jannaschii gene, Mj0226. The crystal structure of Mj0226 protein determined at 2.2 A resolution reveals that the protein is a homodimer and each monomer folds into an elongated alpha/beta structure of a new fold family. Comparisons of Mj0226 protein with protein structures in the database, however, indicate that one part of the protein is homologous to some of the nucleotide-binding proteins. Biochemical analysis shows that Mj0226 protein is a novel nucleotide triphosphatase that can efficiently hydrolyze nonstandard nucleotides such as XTP to XMP or ITP to IMP, but not the standard nucleotides, in the presence of Mg2+ or Mn2+ ions. |
==About this Structure== | ==About this Structure== | ||
| - | 2MJP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with ANP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2MJP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: BSGC, Berkeley | + | [[Category: BSGC, Berkeley Structural Genomics Center.]] |
[[Category: Cho, Y.]] | [[Category: Cho, Y.]] | ||
| - | [[Category: Chung, J | + | [[Category: Chung, J H.]] |
| - | [[Category: Han, Y | + | [[Category: Han, Y S.]] |
| - | [[Category: Hwang, K | + | [[Category: Hwang, K Y.]] |
| - | [[Category: Kim, S | + | [[Category: Kim, S H.]] |
[[Category: ANP]] | [[Category: ANP]] | ||
[[Category: berkeley structural genomics center]] | [[Category: berkeley structural genomics center]] | ||
| Line 28: | Line 28: | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:07:44 2008'' |
Revision as of 16:07, 21 February 2008
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STRUCTURE-BASED IDENTIFICATION OF THE BIOCHEMICAL FUNCTION OF A HYPOTHETICAL PROTEIN FROM METHANOCOCCUS JANNASCHII:MJ0226
Overview
Almost half of the entire set of predicted genomic products from Methanococcus jannaschii are classified as functionally unknown hypothetical proteins. We present a structure-based identification of the biochemical function of a protein with an as yet unknown function from a M. jannaschii gene, Mj0226. The crystal structure of Mj0226 protein determined at 2.2 A resolution reveals that the protein is a homodimer and each monomer folds into an elongated alpha/beta structure of a new fold family. Comparisons of Mj0226 protein with protein structures in the database, however, indicate that one part of the protein is homologous to some of the nucleotide-binding proteins. Biochemical analysis shows that Mj0226 protein is a novel nucleotide triphosphatase that can efficiently hydrolyze nonstandard nucleotides such as XTP to XMP or ITP to IMP, but not the standard nucleotides, in the presence of Mg2+ or Mn2+ ions.
About this Structure
2MJP is a Single protein structure of sequence from Methanocaldococcus jannaschii with as ligand. Full crystallographic information is available from OCA.
Reference
Structure-based identification of a novel NTPase from Methanococcus jannaschii., Hwang KY, Chung JH, Kim SH, Han YS, Cho Y, Nat Struct Biol. 1999 Jul;6(7):691-6. PMID:10404228
Page seeded by OCA on Thu Feb 21 18:07:44 2008
Categories: Methanocaldococcus jannaschii | Single protein | BSGC, Berkeley Structural Genomics Center. | Cho, Y. | Chung, J H. | Han, Y S. | Hwang, K Y. | Kim, S H. | ANP | Berkeley structural genomics center | Bsgc structure funded by nih | Hyperthermal protein | Protein structure initiative | Psi | Pyrophosphatase | Structural genomics
