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2rjf
From Proteopedia
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| - | [[Image:2rjf.png|left|200px]] | ||
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{{STRUCTURE_2rjf| PDB=2rjf | SCENE= }} | {{STRUCTURE_2rjf| PDB=2rjf | SCENE= }} | ||
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===Crystal structure of L3MBTL1 in complex with H4K20Me2 (residues 12-30), orthorhombic form I=== | ===Crystal structure of L3MBTL1 in complex with H4K20Me2 (residues 12-30), orthorhombic form I=== | ||
| + | {{ABSTRACT_PUBMED_18026117}} | ||
| - | + | ==Function== | |
| + | [[http://www.uniprot.org/uniprot/LMBL1_HUMAN LMBL1_HUMAN]] Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.<ref>PMID:17540172</ref> <ref>PMID:18408754</ref> <ref>PMID:20870719</ref> <ref>PMID:20870725</ref> | ||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:018026117</ref><references group="xtra"/> | + | <ref group="xtra">PMID:018026117</ref><references group="xtra"/><references/> |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Allali-Hassani, A.]] | [[Category: Allali-Hassani, A.]] | ||
Revision as of 07:01, 29 September 2013
Contents |
Crystal structure of L3MBTL1 in complex with H4K20Me2 (residues 12-30), orthorhombic form I
Template:ABSTRACT PUBMED 18026117
Function
[LMBL1_HUMAN] Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.[1] [2] [3] [4]
About this Structure
2rjf is a 5 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Min J, Allali-Hassani A, Nady N, Qi C, Ouyang H, Liu Y, MacKenzie F, Vedadi M, Arrowsmith CH. L3MBTL1 recognition of mono- and dimethylated histones. Nat Struct Mol Biol. 2007 Dec;14(12):1229-30. Epub 2007 Nov 18. PMID:18026117 doi:10.1038/nsmb1340
- ↑ Trojer P, Li G, Sims RJ 3rd, Vaquero A, Kalakonda N, Boccuni P, Lee D, Erdjument-Bromage H, Tempst P, Nimer SD, Wang YH, Reinberg D. L3MBTL1, a histone-methylation-dependent chromatin lock. Cell. 2007 Jun 1;129(5):915-28. PMID:17540172 doi:10.1016/j.cell.2007.03.048
- ↑ Kalakonda N, Fischle W, Boccuni P, Gurvich N, Hoya-Arias R, Zhao X, Miyata Y, Macgrogan D, Zhang J, Sims JK, Rice JC, Nimer SD. Histone H4 lysine 20 monomethylation promotes transcriptional repression by L3MBTL1. Oncogene. 2008 Jul 17;27(31):4293-304. doi: 10.1038/onc.2008.67. Epub 2008 Apr, 14. PMID:18408754 doi:10.1038/onc.2008.67
- ↑ Saddic LA, West LE, Aslanian A, Yates JR 3rd, Rubin SM, Gozani O, Sage J. Methylation of the retinoblastoma tumor suppressor by SMYD2. J Biol Chem. 2010 Nov 26;285(48):37733-40. doi: 10.1074/jbc.M110.137612. Epub, 2010 Sep 24. PMID:20870719 doi:10.1074/jbc.M110.137612
- ↑ West LE, Roy S, Lachmi-Weiner K, Hayashi R, Shi X, Appella E, Kutateladze TG, Gozani O. The MBT repeats of L3MBTL1 link set8 mediated p53 methylation at lysine 382 to target gene repression. J Biol Chem. 2010 Sep 24. PMID:20870725 doi:10.1074/jbc.M110.139527
Categories: Homo sapiens | Allali-Hassani, A. | Arrowsmith, C H. | Bochkarev, A. | Crombet, L. | Edwards, A M. | Herzanych, N. | Kozieradzki, I. | Liu, Y. | Loppnau, P. | Mackenzie, F. | Min, J R. | Ouyang, H. | SGC, Structural Genomics Consortium. | Sundstrom, M. | Vedadi, M. | Weigelt, J. | Chromatin regulator | Chromosomal protein | Dna-binding | Metal-binding | Methylation | Nucleosome core | Nucleus | Repressor | Sgc | Structural genomic | Structural genomics consortium | Transcription | Transcription regulation | Zinc-finger
