1w8h

From Proteopedia

Revision as of 14:23, 30 October 2007

STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LEWISA TRISACCHARIDE

Overview

One of the mechanisms contributing to the protection by breast-feeding of, the newborn against enteric diseases is related to the ability of human, milk oligosaccharides to prevent the attachment of pathogenic bacteria to, the duodenual epithelium. Indeed, a variety of fucosylated, oligosaccharides, specific to human milk, form part of the innate immune, system. In the present study, we demonstrate the specific blocking of, PA-IIL, a fucose-binding lectin of the human pathogen Pseudomonas, aeruginosa, by milk oligosaccharides. Two fucosylated epitopes, Lewis a, and 3-fucosyl-lactose (Lewis x glucose analogue) bind to the lectin with, dissociation constants of 2.2x10(-7) M and 3.6x10(-7) M respectively., Thermodynamic studies indicate that these interactions are dominated by, enthalpy. The ... [(full description)]

About this Structure

1W8H is a [Single protein] structure of sequence from [Pseudomonas aeruginosa] with CA, SO4 and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa., Perret S, Sabin C, Dumon C, Pokorna M, Gautier C, Galanina O, Ilia S, Bovin N, Nicaise M, Desmadril M, Gilboa-Garber N, Wimmerova M, Mitchell EP, Imberty A, Biochem J. 2005 Jul 15;389(Pt 2):325-32. PMID:15790314

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