2phr

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(New page: 200px<br /><applet load="2phr" size="350" color="white" frame="true" align="right" spinBox="true" caption="2phr, resolution 1.90&Aring;" /> '''Pterocarpus angolens...)
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==Overview==
==Overview==
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The crystal structure of Pterocarpus angolensis seed lectin is presented, in complex with a series of high mannose oligosaccharides ranging from, Man-5 to Man-9. Despite that several of the nine mannose residues of Man-9, have the potential to bind in the monosaccharide binding site, all, oligomannoses are bound in the same unique way employing the, tetrasaccharide sequence, Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. ITC titration, experiments using Man-5, Man-9 and the Man-9-containing glycoprotein, soybean agglutinin as ligands confirm the monovalence of Man-9 and show a, four times higher affinity for Man-9 when it is presented to Pterocarpus, angolensis seed lectin in a glycoprotein context.
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The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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How a Plant Lectin Recognizes High Mannose oligosaccharides., Garcia-Pino A, Buts L, Wyns L, Imberty A, Loris R, Plant Physiol. 2007 Jun 7;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17556509 17556509]
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How a plant lectin recognizes high mannose oligosaccharides., Garcia-Pino A, Buts L, Wyns L, Imberty A, Loris R, Plant Physiol. 2007 Aug;144(4):1733-41. Epub 2007 Jun 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17556509 17556509]
[[Category: Pterocarpus angolensis]]
[[Category: Pterocarpus angolensis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: lectin]]
[[Category: lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:43:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:29:32 2008''

Revision as of 16:29, 21 February 2008

Image:2phr.jpg

2phr, resolution 1.90Å

Drag the structure with the mouse to rotate

Pterocarpus angolensis lectin (PAL) in complex with Man-7D1

Overview

The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.

About this Structure

2PHR is a Single protein structure of sequence from Pterocarpus angolensis with and as ligands. Full crystallographic information is available from OCA.

Reference

How a plant lectin recognizes high mannose oligosaccharides., Garcia-Pino A, Buts L, Wyns L, Imberty A, Loris R, Plant Physiol. 2007 Aug;144(4):1733-41. Epub 2007 Jun 7. PMID:17556509

Page seeded by OCA on Thu Feb 21 18:29:32 2008

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